Addgene

We narrowed to 2,305 results for: TIR

Showing: 101 - 110 of 2305 results

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Use

Tags

SNAP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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PADH1-TIR1-9myc-URA3

Plasmid

#207016

Purpose

Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

TIR1

Use

Tags

9xMyc

Expression

Yeast

Mutation

Promoter

pADH1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-T2A-H2B mCitirine

Plasmid

#210471

Purpose

Coexpression of Cas9 and H2B mCitrine

Depositor

Amro Hamdoun

Insert

Cas9-T2A-H2B mCitirine

Use

CRISPR and Synthetic Biology

Tags

mCitrine on C terminal of H2B

Expression

Mammalian

Mutation

Cas9 contains 3xFLAG tag and nuclear localization…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIRSTD)-mEos3.2

Plasmid

#189719

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD/HisB

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Use

Tags

6MYC

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6FLAG osTIR1

Plasmid

#182515

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Use

Tags

6FLAG

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-mNG

Plasmid

#189722

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIR23)-sfGFP

Plasmid

#189676

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

HTA1

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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