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pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp
Plasmid#185061PurposeEf1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445DepositorInsertU6:pegRNA:scaffold:PBS+RT template
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE
Plasmid#188539PurposeAn AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.DepositorInsertsecreted EGFP
UseAAVTagsCDNF(1-28) and THPKTEL WPREExpressionMutationPromoterCMV-IEAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Akaluc VLP
Plasmid#182212PurposeYIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-Akaluc
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c020
Plasmid#175484PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Eva1b
Plasmid#175158PurposeLentiviral expression of V5-tagged mouse Eva1bDepositorInsertEva1b (Eva1b Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Cenpv-V5
Plasmid#175125PurposeLentiviral expression of mouse Cenpv-V5DepositorInsertCenpv (Cenpv Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
L-SACSIN-74
Plasmid#166526PurposescFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-FHL1-1
Plasmid#166543PurposescFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
C-BRPF3-8
Plasmid#166516PurposescFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR2F1-donor
Plasmid#112388PurposeCRISPR donor plasmid to tag human transcription factor NR2F1 with GFPDepositorInsertNR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
UseCRISPRTagsExpressionMutationrs974916569PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF250-donor
Plasmid#112383PurposeCRISPR donor plasmid to tag human transcription factor ZNF250 with GFPDepositorInsertZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
UseCRISPRTagsExpressionMutationhg38:8:144882271:C>G,hg38:8:144882273:T>CPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF140-donor
Plasmid#112363PurposeCRISPR donor plasmid to tag human transcription factor ZNF140 with GFPDepositorInsertZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)
UseCRISPRTagsExpressionMutationhg38:12:133105834:A>G, hg38:12:133105852:A>GPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF639-donor
Plasmid#112345PurposeCRISPR donor plasmid to tag human transcription factor ZNF639 with GFPDepositorInsertZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
UseCRISPRTagsExpressionMutationhg38:3:179333542:G>T,rs978050614PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBPL1-donor
Plasmid#112316PurposeCRISPR donor plasmid to tag human transcription factor TBPL1 with GFPDepositorInsertTBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
UseCRISPRTagsExpressionMutationrs35776966, hg38:6:133987646:GTGTAT>-PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKN2 (PRKCL2A-c038)
Plasmid#110265PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPRKCL2 (PKN2 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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psicheck_NDUFA6_3UTR_mut
Plasmid#65971PurposeLuciferase assay vector, assessing regulation of NDUFA6 3' UTR, with two mutated tandem CAC motifsDepositorInsertNDUFA6 (NDUFA6 Human)
UseTagsExpressionMammalianMutationmutated tandem CAC motif to CCC motifPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only
