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Plasmid#207855PurposeMammalian expression of PE6e prime editorDepositorInsertPE6e
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, K918A, K775R)PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-PM
Plasmid#125122PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-PM
UseTagsIg-K-Chain and membrane anchoring sequence-PDGFRExpressionMammalianMutationNAPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
7M8
Plasmid#64839PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.DepositorInsert7M8 cap
UseAAVTagsExpressionMutation7mer insertion in the AAV2 capPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmaxRNaseHtrunc
Plasmid#207858PurposeMammalian expression of PEmaxDRNaseH prime editorDepositorInsertPEmaxDRNaseH
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationM-MLVRTDRNAseHrtPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP5380 LAMP1-SBP- mCherry-FIREtag
Plasmid#216725PurposeExpresses LAMP1-mCherry-FIREtag in mamallian cellsDepositorInsertLAMP-1 (LAMP1 Human)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB1
Plasmid#35535DepositorInsert3'UTR of ZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Rtn4a
Plasmid#86683PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertRtn4a (RTN4 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA8
Plasmid#177661PurposeRecombinant protein synthesis of human HSPA8DepositorInsertHSPA8 (HSPA8 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA8
Plasmid#19514DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Mito-SBFP2
Plasmid#187964PurposeMammalian cell expression of a fluorescent SBFP2-tagged mitochondrial markerDepositorInsertMito (COX8A Human)
UseTagsSBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-synaptoPAC-minWPRE
Plasmid#153100PurposeCre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neuronsDepositorInsertsUseAAVTagsHis tag, mScarlet, and myc tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM-mut
Plasmid#187152Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UIM-mut
Plasmid#187148Purposemammalian cell expression of FLAG-HUWE1 A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationA1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184741Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE050
Plasmid#176807PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert601
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI
Plasmid#184742Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE060
Plasmid#176817PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert611
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI2 (GNAI2 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)QPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE065
Plasmid#176822PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert704
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only