Addgene

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Showing: 2141 - 2160 of 9057 results

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pENTR-EGFP-TEADiv2

Plasmid

#228874

Purpose

Gateway entry vector for optimized GFP-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Gateway entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Use

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5(PrD)

Plasmid

#166167

Purpose

Gateway entry vector for N. furzeri DDX5-PrD(535-634) mutant

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX5-PrD(535-634)

Use

Gateway entry vector

Tags

Expression

Mutation

PrD only 1606-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5ΔPrD

Plasmid

#166164

Purpose

Gateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutant

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX5ΔPrD(1-535)

Use

Gateway entry vector

Tags

Expression

Mutation

deletion of 1606-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5ΔIDR

Plasmid

#166165

Purpose

Gateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutant

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX5ΔIDR(1-483)

Use

Gateway entry vector

Tags

Expression

Mutation

deletion of 1450-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5(IDR)

Plasmid

#166166

Purpose

Gateway entry vector for N. furzeri DDX5-IDR(483-634) mutant

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX5-IDR(483-634)

Use

Gateway entry vector

Tags

Expression

Mutation

IDR only 1450-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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MBD-IFP2.0C

Plasmid

#215748

Purpose

Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Use

Unspecified

Tags

Expression

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR

Plasmid

#208328

Purpose

MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

H2B-MiniR

Use

Tags

MiniR

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-SOS

Plasmid

#208278

Purpose

Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-SOS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-EGFR

Plasmid

#208281

Purpose

Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-EGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TinyERRE

Plasmid

#208324

Purpose

C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TinyERRE

Use

Tags

TinyERRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-mCh-bla

Plasmid

#210260

Purpose

Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Use

Tags

PunyEEER

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Annexin A7-TEV-TwinStrep

Plasmid

#198635

Purpose

Annexin A7 fibrillization and lipid binding

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 (ANXA7 Human)

Use

Tags

TEV-GS-Dual Strep

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-luciferase

Plasmid

#198590

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

luciferase

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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