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Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(0)
Plasmid#164269PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PTEN 3'UTR
Plasmid#28104DepositorInsertPTEN 3'UTR (Pten Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K840R
Plasmid#204418PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
UseTagsHA and YFPExpressionMammalianMutationLysine 840 to Arginine (K840R)PromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K1097R
Plasmid#204417PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
UseTagsHA and YFPExpressionMammalianMutationLysine 1097 to Arginine (K1097R)PromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rpp38-C1
Plasmid#134548PurposeExpresses YFP-Rpp38 fusion proteinDepositorInsertRpp38 (RPP38 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TKGAPDH in pLJM60
Plasmid#154217PurposeLentiviral vector for stable expression of FLAG-TK-GAPDHDepositorInsertGAPDH
UseLentiviralTagsFLAGExpressionMammalianMutationcDNA has been codon optimized for human cell expr…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1 S449A/T450A/S454A
Plasmid#19847DepositorInsertMKL1 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGExpressionMutationchanged Serine 449, Threonine 450 and Serine 454 …PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCF708
Plasmid#121685PurposeEF1a-dTEV-protease-mTagBFP2DepositorInsertTEV protease
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rpp25-C1
Plasmid#134544PurposeExpresses YFP-Rpp25 fusion proteinDepositorInsertRpp25 (RPP25 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S454A
Plasmid#19844DepositorInsertMKL1 S454A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 454 to AlaninePromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
eTC GFP ß-actin ΔZip
Plasmid#27124DepositorInsertbeta-actin (ACTB Human)
UseTagsEGFP and TC tagExpressionMammalianMutationThis construct lacks β-actin 3′UTR and therefore …PromoterAvailable sinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-Sgo1 N61I
Plasmid#108496Purposeexpression of vsv-Sgo1 N61I (no PP2A binding)DepositorInsertShugoshin 1 (SGO1 Human)
UseTagsVSVExpressionMammalianMutationno PP2A binding N61IPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXL001
Plasmid#26122DepositorInserttTR-KRAB
UseLentiviralTagsIRES-PuroExpressionMutationPromoterAvailable sinceJune 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rpp20-C1
Plasmid#134541PurposeExpresses YFP-Rpp20 fusion proteinDepositorInsertRpp20
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p156 pWZL-EGFP-SLPI
Plasmid#11069DepositorInsertSLPI (Slpi Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pNC1136
Plasmid#41562DepositorInsertUra3-FUS1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
JP1520 PIK3CA H1047R HA
Plasmid#14572DepositorInsertPIK3CA H1047R (PIK3CA Bovine)
UseRetroviralTagsHA tagExpressionMammalianMutationH1047RPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only