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We narrowed to 8,045 results for: sgRNA

Showing: 2141 - 2160 of 8045 results

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pLY171

Plasmid

#184145

Purpose

sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

sgRNA-LEA2-ex3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Plux2

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pA-RFP-rG2

Plasmid

#188969

Purpose

IPTG inducible mCherry with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

mCherry
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Cquin_728

Plasmid

#176656

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Agam_557

Plasmid

#176667

Purpose

Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_972

Plasmid

#176674

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Agam_557

Plasmid

#176653

Purpose

Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aalb_726

Plasmid

#176675

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_763

Plasmid

#176672

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_972

Plasmid

#176660

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Cquin_801

Plasmid

#176657

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_801

Plasmid

#176671

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_728

Plasmid

#176670

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK_1

Plasmid

#163462

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 1 targeting NADK (NADK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKH-1699

Plasmid

#124227

Purpose

Mammalian SpCas9-HF1 expression

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

SpCas9-HF1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(G:H)

Plasmid

#138257

Purpose

Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

GFP targeting sgRNAs G and H

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hsg(G:H)

Plasmid

#138259

Purpose

Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mCherry targeting sgRNAs G and H

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-SENP8 sg2R-P2A-Hygro

Plasmid

#124300

Purpose

Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

SENP8 (SENP8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-2

Plasmid

#86135

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-3

Plasmid

#86136

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 3 targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge