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Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCSGC-GB1
Plasmid#186786PurposeE. coli expression vector containing N-terminal 6His-GB1-TEV fusion.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-RCC1-polyA
Plasmid#59762PurposeExpress Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRCC1
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x2
Plasmid#63602PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2
Plasmid#51585Purposeexpresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)DepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x4
Plasmid#63604PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZLSLD.R
Plasmid#51511PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x7
Plasmid#63607PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S129N
Plasmid#51587Purposeexpresses RNAiR myc-tagged Rem2 non-GTP bindingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationS129N; silent mutations at shRNA 1 and 2 recognit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 L317G
Plasmid#51586Purposeexpresses RNAiR myc-tagged Rem2 non-CaM interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationL317G; silent mutations at shRNA 1 and 2 recognit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
B3rec.zf1
Plasmid#108354PurposeZebrafish codon optimized B3 recombinase that acts on blown-out sitesDepositorInsertB3 recombinase
UseSynthetic BiologyTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x6
Plasmid#63606PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x3
Plasmid#63603PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D
Plasmid#51496PurposepLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmidDepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG
Plasmid#217725PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-179PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-HT-emiRFP703
Plasmid#183985PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.DepositorInsertH2B (H2BC11 Human)
UseTagsHaloTag, emiRFP703ExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-HT-mGold
Plasmid#183988PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.DepositorInsertH2B (H2BC11 Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Isce-Tol2-Tn5-neo
Plasmid#176619Purposefor generating PCR templates with Tol2 repeats and Isce sites for BAC recombinationDepositorInsertI-SceI sites, Tol2 repeats and Tn5-neo cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only