Addgene

We narrowed to 11,984 results for: lic

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pPBAD-yhjH

Plasmid

#111613

Purpose

Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

Depositor

Tim Tolker-Nielsen

Article

Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

Insert

yhjH

Use

Tags

Expression

Bacterial

Mutation

A52V

Promoter

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfp(ASV)C

Plasmid

#111617

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfpC

Plasmid

#111616

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40731

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1 S449A/T450A/S454A

Plasmid

#19847

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pSG5-ERRbeta2

Plasmid

#52186

Purpose

Expresses human ERRbeta2 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Use

Tags

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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MCV-Rep-

Plasmid

#32058

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-Rep- genome

Use

Tags

Expression

Mutation

Single cytosine to adenine (C/A) mutation at 2,07…

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

Plasmid

#133333

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All W to S in CTD of full-length TDP43

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Article

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBIND-HBSP

Plasmid

#53113

Purpose

Expression of GAL4BD-tagged Hepatitis B splicing protein

Depositor

Xu Lin

Article

Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

Insert

HBSP

Use

Tags

GAL4BD

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only
pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

J23101

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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GST-SmB'

Plasmid

#1842

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB' (SNRPB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2

Plasmid

#183744

Purpose

Expresses Ki67 (shuffled Nterm)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (shuffled Nterm) (MKI67 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

CD domain with shuffled 4 fragments fused to the …

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1410 GFP-TDP43 F-S

Plasmid

#118799

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All F to S in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj10a-IRES-EGFP

Plasmid

#164947

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj10a (kcnj10a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 S449A/T450A/S454A

Plasmid

#19845

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

Plasmid

#118804

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GST-SmB

Plasmid

#1841

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB (SNRPB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pUB6_EPB49

Plasmid

#84015

Purpose

EPB49 minigene to test splicing event

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

EPB49 (DMTN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UB6

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D444-500

Plasmid

#19839

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 D444-500 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-500

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only