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Showing: 2101 - 2120 of 4770 results

  4. lentiCRISPR - CTRL sgRNA

    Plasmid
    #70662
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    non-targeting sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70662-plasmid-map-sequence-id-162511

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  15. p5T7-LYCipi-ggpps

    Plasmid
    #122017
    Purpose
    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Insert
    GGPP synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    codon optimized, truncated first 98 amino acids, …
    Promoter
    T7
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122017-plasmid-map-sequence-id-238964

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  16. pET-APOBEC1-YTHmut

    Plasmid
    #200159
    Purpose
    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTHmut
    Use
    Tags
    6xHis, HA, and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200159-plasmid-map-sequence-id-396325

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  20. pBJG1 T7-8xHis-OGT

    Plasmid
    #154271
    Purpose
    Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    bacterial codon optimized
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154271-plasmid-map-sequence-id-304821

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Showing: 2101 - 2120 of 4770 results