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Plasmid#107473PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transportDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationI576VPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTL550V-myc
Plasmid#107472PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transportDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationL550VPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-BLM-UGI-T2A-mTagBFP2
Plasmid#221222PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporterDepositorInsertAID-BLM-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-TET2Puro-gal4DBD-miniVPR-HA
Plasmid#207169PurposeDox Inducible Expression Plasmid for gal4DBD-miniVPR-HADepositorInsertGal4DBD-miniVPR
UseLentiviral and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cyto-APEX2
Plasmid#215552PurposeStable, inducible expression in cytoplasmDepositorInsertEGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Endo-APEX2
Plasmid#215553PurposeStable, inducible expression in endosomeDepositorInsertEGFP-2xFYVE-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP
Plasmid#213517PurposeExpresses human KU70-eGFP in mammalian cellsDepositorInserthuman KU70-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-APEX2
Plasmid#215555PurposeStable, inducible expression in lysosomeDepositorInsertLAMP1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-tmpknot-epegRNA-puro backbone
Plasmid#214089PurposeLentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dsup pLVX-Puro
Plasmid#180635PurposeExpresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio toleranceDepositorInsertDsup
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP
Plasmid#221233PurposeExpress sgRNA targeting HEK3 loci with nCas9(D10A)DepositorInsertSpCas9(D10A)-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybGam-WPRE
Plasmid#207662PurposeControl for CybSEP2DepositorInsertGamillus
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted gamill…PromoterhSynapsinAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast
Plasmid#206217PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL
Plasmid#226517PurposeMamalian cell expression of SNAP-tag2 in endoplasmic reticulumDepositorInsertCalR-SNAP-tag2-KDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9(H840A)-T2A-EGFP
Plasmid#221231PurposeExpress nCas9 nickase (H840A) with GFP reporterDepositorInsertSpCas9(H840A)-T2A-EGFP
UseTagsExpressionMammalianMutationH840APromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-LNS2
Plasmid#220083Purposehigh affinity PA biosensor with green fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2m
Plasmid#208686PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only