We narrowed to 12,315 results for: LEA
-
Plasmid#104561PurposeExpress Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pLX209-neo-WRN E84A K577M
Plasmid#125791Purposeconstitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRNDepositorInsertmyc-WRN E84A/K577M (WRN Human)
Tags5x mycExpressionMammalianMutationE84A/K577M (exonuclease/helicase-dead)Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-7 aSyn C141
Plasmid#108866PurposeExpresses human WT alpha synuclein with a C-terminal cysteineDepositorInsertalpha synuclein (SNCA Human)
ExpressionBacterialMutationC141 mutation to allow C-terminal maleimide dye l…PromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Delta(Pro-MP)ADAM17-HA
Plasmid#65221PurposeExpression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cellsDepositorInsertDelta(Pro-MP)ADAM17 (ADAM17 Human)
TagsHAExpressionMammalianMutationdeleted AA 18-473PromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARIO/ pcDNA3
Plasmid#124838Purposemammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMARIO
TagsNo tagExpressionMammalianPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-hHelios-IRES-GFP
Plasmid#74047Purposeretroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1DepositorInserthuman Helios (IKZF2) (IKZF2 )
UseRetroviralTagsFLAGExpressionMammalianMutationencodes shorter version of human HeliosPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-s-pep
Plasmid#47438PurposeFRET sensor for beta2-adrenergic receptorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…ExpressionMammalianPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcullin 1 K720A (CUL1 Human)
TagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGJK_His-SUMO-LbuCas13a
Plasmid#172488PurposeBacterial expression of codon-optimized His-SUMO-LbuCas13a.DepositorInsertbdSUMO-LbuCas13a (codon-optimized)
TagsHis6 and bdSUMOExpressionBacterialPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRT.Agro.CLCrVA.008
Plasmid#31809DepositorInsertJRTCLCrVA.008
UseAgrobacterium binaryAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_Ado1.0med
Plasmid#153286PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neuronsDepositorHas serviceAAV2InsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVPromoterhSynAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET11d-RAN
Plasmid#108919PurposeExpresses human RANDepositorInsertRAN (RAN Human)
ExpressionBacterialAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-ISceID44A
Plasmid#59424PurposeExpresses a catalytically inactive form of the endonuclease ISceIDepositorInsertendonuclease ISceI mutant (I-SceI )
TagsHA tagExpressionMammalianMutationchanged aspartate 44 to alanine (D44A)Available sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTrcHisA-PPARalpha(aa101-468)
Plasmid#13574DepositorInserthuman peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
Tagsx6His N-terminal, enterokinase cleavage siteExpressionBacterialMutationdeleted residues 1-100.Available sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
Plasmid#154787PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domainDepositorInsertAdenosine deaminse (Adar Synthetic, Fly)
UseLentiviralTagsGFP, V5, and stdMCPExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR2.1 Clover-LMNA Donor
Plasmid#122508PurposeHomology repair template for in frame (first exon) clover knock-in of human LMNA geneDepositorInsertHomology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
UseHomology repair template plasmid (donor plasmid)TagsCloverAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2T-CMV-ABEmax-BlastR
Plasmid#152989PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE7.10-nSpCas9
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by