We narrowed to 12,117 results for: LEA
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Plasmid#107934PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neuronsDepositorAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLenti_CamKIIa_mKate2
Plasmid#96942PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertfar-red fluorescent protein
UseLentiviralMutationE87D (please see depositor comments below)PromoterCaMKIIaAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA
Plasmid#178534PurposeModifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)DepositorTypeEmpty backboneTagsHAExpressionMammalianPromoterEF1aAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianPromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7goldRNA
Plasmid#47930PurposeIn vitro transcription of golden gRNADepositorInsertgolden gRNA (slc24a5 Zebrafish)
UseCRISPRAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED
Plasmid#154088PurposeImproved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.DepositorUseLentiviralExpressionMammalianPromoterTet onAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-YAP-deltaC
Plasmid#21126DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
TagsEGFP-C3ExpressionBacterial and MammalianMutation5 Carboxy-terminal amino acids -FLTWL are deletedAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cdc2 (716)
Plasmid#61840Purposemammalian expression of cdc2DepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16
Plasmid#172746PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-16
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
Plasmid#183790PurposeA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.DepositorInsertsP2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC
UseSynthetic BiologyMutationG>A in the gRNA scaffold- please see depositor…Available SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-p38beta
Plasmid#86833Purposemammalian expression of GFP-p38betaDepositorAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV/N3IC-HA
Plasmid#47618Purposein vitro transcription and translation of HA-tagged Notch3 Intracellular domainDepositorInsertNotch3 Intracellular domain (Notch3 Mouse)
UseIn vitro transcription and translationTagsHAExpressionMammalianMutationcodons 1664 (Met) to 2318 (Ala)PromoterCMVAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG545
Plasmid#40116DepositorTypeEmpty backboneUseGateway CloningTagsDendra2 PAFP (sequence optimized for worm express…Available SinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTALEN_v2 (NN)
Plasmid#32191DepositorInsertTALE Nuclease, NN for 0.5 repeat
ExpressionMammalianAvailable SinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN E84A K577M
Plasmid#125791Purposeconstitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRNDepositorInsertmyc-WRN E84A/K577M (WRN Human)
Tags5x mycExpressionMammalianMutationE84A/K577M (exonuclease/helicase-dead)Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(WT)
Plasmid#84639PurposemCherry-tagged, wild-type LKB1DepositorInsertmCherry-LBK1 (STK11 Human, Synthetic)
Tags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianPromoterCMVAvailable SinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS tdTomato
Plasmid#91767PurposeBacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)DepositorInserttandem dimer Tomato
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II 31-52
Plasmid#35179DepositorInsertRPBI (POLR2A Human)
TagsFLAGExpressionMammalianMutationDeletion of C-terminal domain repeats 1-30PromoterCMVAvailable SinceOct. 2, 2012AvailabilityAcademic Institutions and Nonprofits only