We narrowed to 16,071 results for: LEA

1. Emerin pEGFP-C1 (637)

   Plasmid

   #61993

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Emerin pEGFP-N2 (588)

   Plasmid

   #61985

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. NET5s pEGFP-N2 (1154)

   Plasmid

   #62034

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET5s (TMEM201 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   short form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. NET5L pEGFP-N2 (1155)

   Plasmid

   #62035

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   NET5L (TMEM201 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   long form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLV-REPORT(EF1a)-TTN

   Plasmid

   #172327

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cp PS Intein dCas9-VPR

   Plasmid

   #242031

   Purpose

   Blue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

   Insert

   Cp PS Intein dCas9-VPR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_PCP-T5Exo

   Plasmid

   #214736

   Purpose

   encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_PCP-T5Exo_bGH
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. nesprin TSmod 25

   Plasmid

   #125555

   Purpose

   Nesprin-2G force sensor with cerulean-F25-venus TSmodule

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   mini-nesprin-2G (Syne2 Mouse)
   

   Tags

   cerulean, venus

   Expression

   Mammalian

   Mutation

   TSmod changed from mTFP to cerulean, flageliform …

   Promoter

   CMV

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE4398

    Plasmid

    #74042

    Purpose

    Expresses human codon-optimized LbCpf1 and Lb crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Lb crRNA
    LbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExpreS2-1-C3-10H-SIII

    Plasmid

    #175444

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-10His-TwinStrep and BiP Secretion Peptide

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS CMV GAG

    Plasmid

    #234992

    Purpose

    For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    GAG
    

    Expression

    Mammalian

    Mutation

    Deleted polymerase coding sequence

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Nuc-ExRai-AktAR2

    Plasmid

    #184049

    Purpose

    Nuclear-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Nuc-ExRai-AktAR2
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDuBir-Lbu-dCas13a-avitag

    Plasmid

    #100817

    Purpose

    Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

    Depositor

    Mitchell O'Connell

    Article

    Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

    Insert

    Lbu_Cas13a_R472A_H477A_R1048A_H1053A
    

    Tags

    AviTag and His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    T7

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGT9050

    Plasmid

    #219429

    Purpose

    PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AATG_PapE-4xLF2_AGGT
    

    Use

    Moclo compatible level 0 module

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tmem38A pEGFP-N2 (1401)

    Plasmid

    #62050

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem38A (TMEM38A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMSCV-BirA-P2A-mCherry

    Plasmid

    #74056

    Purpose

    retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    BirA biotin ligase
    

    Use

    Retroviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Promoter

    MSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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