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Addgene
Showing: 2101 - 2120 of 14062 results

  1. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  4. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

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  6. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  12. Monomer NK

    Plasmid
    #31178
    Depositor
    Feng Zhang
    Insert
    Monomer-NK
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. pICSL30017

    Plasmid
    #174583
    Purpose
    Level 0 N-terminal tag module carrying 6xHIS uncleavable
    Depositor
    Mark Banfield
    Insert
    6xHIS uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    174583-plasmid-map-sequence-id-344729

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  14. pICSL50025

    Plasmid
    #174589
    Purpose
    Level 0 C-terminal tag module carrying 6xHIS uncleavable
    Depositor
    Mark Banfield
    Insert
    6xHIS uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    174589-plasmid-map-sequence-id-344730

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  15. pEM8313

    Plasmid
    #213986
    Purpose
    PrpsM driven expression of mCerulean
    Depositor
    Marc Erhardt
    Insert
    mCerulean
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrpsM
    Availability
    Academic Institutions and Nonprofits only
    213986-plasmid-map-sequence-id-424923

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  16. pICSL30022

    Plasmid
    #174586
    Purpose
    Level 0 N-terminal tag module carrying Twin Streptavidin uncleavable
    Depositor
    Mark Banfield
    Insert
    Twin Streptavidin uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    174586-plasmid-map-sequence-id-344732

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  17. pICSL50027

    Plasmid
    #174591
    Purpose
    Level 0 C-terminal tag module carrying Twin Streptavidin uncleavable
    Depositor
    Mark Banfield
    Insert
    Twin Streptavidin uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    174591-plasmid-map-sequence-id-344734

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  18. pEM7065

    Plasmid
    #214003
    Purpose
    PrpsM driven expression of ssrA-tagged mCerulean (LVA)
    Depositor
    Marc Erhardt
    Insert
    mCerulean-LVA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrpsM
    Availability
    Academic Institutions and Nonprofits only
    214003-plasmid-map-sequence-id-424946

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  19. pEM7074

    Plasmid
    #214012
    Purpose
    PrpsM driven expression of ssrA-tagged mCerulean (ASV)
    Depositor
    Marc Erhardt
    Insert
    mCerulean-ASV
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrpsM
    Availability
    Academic Institutions and Nonprofits only
    214012-plasmid-map-sequence-id-424936

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  20. pMal-mRuby3

    Plasmid
    #209348
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209348-plasmid-map-sequence-id-419546

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Showing: 2101 - 2120 of 14062 results