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Plasmid#44566Purposefor generation of knockout of mycobacterial genesDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
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pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntr_PE2
Plasmid#149548PurposeGateway entry clone with PE2 enzyme (with stop codon)DepositorInsertPE2
UseCRISPR; Gateway shuttle vectorTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-M
Plasmid#26589DepositorInsertn/a
UseShuttle vector for mycobacteriaTagsExpressionMutationPromoterAvailable sinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYHY3
Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialMutationPromoterP1TDP-A21Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-GFP-Ubc9wt
Plasmid#20368DepositorInsertSUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-Ub-G76A-Rnq1-GFP
Plasmid#21052DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseYeast/bacteria shuttleTagsGFP and ubiquitinExpressionYeastMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBigTB
Plasmid#149432Purposeshuttle vector with blasticidin-S resistance into ROSA26 targetting vectorsDepositorTypeEmpty backboneUseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2B
Plasmid#118228PurposeEmpty E. coli - B. burgdorferi shuttle vector, blasticidin S resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGU
Plasmid#139933PurposeM. magneticum/E. coli shuttle vector (pMGT and pUC)DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23101Available sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Sen1
Plasmid#99994PurposeShuttle yeast Sen1 into Gateway destination vectorDepositorInsertSen1 (SEN1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBUN276
Plasmid#60673Purposeoverexpression of M. tuberculosis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorTags6 codons of M. bovis BCG hsp60ExpressionMutationPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB72
Plasmid#226312PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1
UseTagsExpressionBacterialMutationY300APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB44
Plasmid#226303PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S MR1
UseTagsExpressionBacterialMutationC292S, C310SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB43
Plasmid#226302PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertWT CsoS2, frameshift site intact
UseTagsExpressionBacterialMutationPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM1
Plasmid#225288PurposeE. coli- C. coli shuttle vector; KanRDepositorInsertReplication elements from pWestmill 11
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only