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Addgene
Showing: 2081 - 2100 of 25717 results
  1. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid
    #154873
    Purpose
    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1
    Depositor
    Insert
    PfROM1 (PF3D7_0506900 Synthetic)
    Use
    Tags
    triple HA
    Expression
    Mammalian
    Mutation
    Alanine 144 changed to Glycine and Asparagine 145…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

    Plasmid
    #108378
    Purpose
    FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Insert
    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

    Plasmid
    #108377
    Purpose
    FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2081 - 2100 of 25717 results