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Plasmid#69936PurposeThe second of the two RNA lassos required for the RNA chain construction.DepositorInsertLasso 2
UseSynthetic BiologyTagsExpressionMutationPromoterTAATACGACTCACTATAGGGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
AL562-2
Plasmid#69937PurposeConstruct targeting the PIE-target sequence.DepositorInsertAL562-2
UseSynthetic BiologyTagsExpressionMutationPromoterTAATACGACTCACTATAGGGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMJ131
Plasmid#69520PurposepBAD HochAld1-20- gfpmut2- ssrADepositorInsertGFPmut2
UseTagsExpressionMutationPromoterAvailable sinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMJ137
Plasmid#69521PurposepBAD etuAld+Ile1-21-gfpmut2- ssrADepositorInsertGFPmut2
UseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMJ130
Plasmid#69519PurposepBAD grpAld1-20- gfpmut2-ssrADepositorInsertGFPmut2
UseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMJ129
Plasmid#69518PurposepBAD etuAld1-20- gfpmut2-ssrADepositorInsertGFPmut2
UseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMJ038
Plasmid#69515PurposepBAD pduD1-20-gfpmut2-ssrADepositorInsertGFPmut2
UseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD30
Plasmid#63691PurposepCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.DepositorInsertV. cholera chromosomal DNA
UseTagsExpressionBacterialMutation540 bp of CT A is deletedPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL2184
Plasmid#71209PurposepbuE Sense Expression PlasmidDepositorInsertpbuE Sense
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJBL1864
Plasmid#71210PurposeAnti-anti Sense Expression PlasmidDepositorInsertAnti-anti Sense
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pSEVA351-Cpf1
Plasmid#192361PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72214-BBa_J72090
Plasmid#40782DepositorInsertVioABCDE
UseSynthetic Biology; PhagemidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-mCherry
Plasmid#123215PurposeN-terminal mCherry tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-FLAG-dN6-His
Plasmid#22143DepositorInsertFlag-clpXdeltaNLinkedHexamer-His6
UseTagsFlag, His6, and L20 LinkersExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-04
Plasmid#187839PurposeSuicide vector to generate markerless gene deletions in Fusobacterium nucleatumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9- AsiA_m2.1
Plasmid#158065PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 2.1DepositorInsertdCas9-AsiA_m2.1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZE31-DsRed-ms2x2
Plasmid#26248DepositorInsertms2 binding sites (x2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only