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Plasmid#70224PurposeExpresses NLS-dCas9-GFP11x7-NLS in mammalian cellsDepositorInsertNLS-dCas9-GFP11x7-NLS
UseTagsGFP11x7ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-VP64-dCas9-VP64-ZsG
Plasmid#192668PurposeDox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1DepositorInsertVP64-dCas9-VP64, ZsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterTRE3GAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry
Plasmid#159655PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLGR002 LGR Cas9 guide vector
Plasmid#188320PurposeUniversal backbone for CRISPR/Cas9 sgRNA expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsPuroR-T2A-mTagBFP2ExpressionMammalianMutationPromoterpU6 5'CMV and EF1-alphaAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9-T2A-mClover3
Plasmid#172293PurposeIn vitro transcription of Cas9-T2A-mClover3 synthetic constructDepositorInsertCas9-T2A-mClover3
UseCRISPRTagsExpressionMutationPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgNts
Plasmid#159909PurposeMutagenesis of NtsDepositorInsertNts (Nts Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-FLAG-dCas9-VPR
Plasmid#114196PurposeLentivirus compatible dCas9-VPR construct driven by the human SYN promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSYNAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9(H840A)-T2A-EGFP
Plasmid#221231PurposeExpress nCas9 nickase (H840A) with GFP reporterDepositorInsertSpCas9(H840A)-T2A-EGFP
UseTagsExpressionMammalianMutationH840APromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VP64-Puro
Plasmid#99371Purpose3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)DepositorInsertdCas9-VP64-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro
Plasmid#192664Purpose3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance markerDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS-GFP-VP16
Plasmid#183923PurposeVP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-VP16
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
GCN5 core-dCas9-p300 core
Plasmid#179553Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CjCas9-eGFP-HIF1a
Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAV and CRISPRTagsSV40NLS-HA-T2A-EGFPExpressionMutationPromoterEF-1alphaAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd2
Plasmid#159918PurposeMutagenesis of Drd2DepositorInsertDrd2 (Drd2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-UCOE-dCas9-BFP-VP64
Plasmid#196717PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sorting.DepositorInsertdCas9-TagBFP-VP64
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationPromoterPtetAAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL7 lenti-dcas9-3xflag-blast
Plasmid#112133Purposelenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)DepositorInsertdCas9(D10A, N863A)-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A mutants in Cas9PromoterEF1aAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only