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Showing: 2061 - 2080 of 48752 results

  1. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

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  2. pcDNA3.2-V5-DEST-hSFRP1-GST

    Plasmid
    #189543
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189543-plasmid-map-sequence-id-374107

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  3. pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

    Plasmid
    #188539
    Purpose
    An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.
    Depositor
    Brandon Harvey
    Insert
    secreted EGFP
    Use
    AAV
    Tags
    CDNF(1-28) and THPKTEL WPRE
    Expression
    Mutation
    Promoter
    CMV-IE
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188539-plasmid-map-sequence-id-370039

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  4. Akaluc VLP

    Plasmid
    #182212
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-Akaluc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182212-plasmid-map-sequence-id-357717

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  5. SDCBPA-c020

    Plasmid
    #175484
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175484-plasmid-map-sequence-id-353208

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  6. pLV V5-Eva1b

    Plasmid
    #175158
    Purpose
    Lentiviral expression of V5-tagged mouse Eva1b
    Depositor
    Larry Gerace
    Insert
    Eva1b (Eva1b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175158-plasmid-map-sequence-id-352269

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  7. pLV Cenpv-V5

    Plasmid
    #175125
    Purpose
    Lentiviral expression of mouse Cenpv-V5
    Depositor
    Larry Gerace
    Insert
    Cenpv (Cenpv Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175125-plasmid-map-sequence-id-351968

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  8. L-SACSIN-74

    Plasmid
    #166526
    Purpose
    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166526-plasmid-map-sequence-id-329139

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  9. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166543-plasmid-map-sequence-id-328279

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  10. C-BRPF3-8

    Plasmid
    #166516
    Purpose
    scFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166516-plasmid-map-sequence-id-328228

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  11. pNR2F1-donor

    Plasmid
    #112388
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR2F1 with GFP
    Depositor
    Kevin White
    Insert
    NR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs974916569
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112388-plasmid-map-sequence-id-229852

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  12. pZNF250-donor

    Plasmid
    #112383
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF250 with GFP
    Depositor
    Kevin White
    Insert
    ZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:144882271:C>G,hg38:8:144882273:T>C
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112383-plasmid-map-sequence-id-229838

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  13. pZNF140-donor

    Plasmid
    #112363
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF140 with GFP
    Depositor
    Kevin White
    Insert
    ZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:133105834:A>G, hg38:12:133105852:A>G
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112363-plasmid-map-sequence-id-229689

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  14. pZNF639-donor

    Plasmid
    #112345
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF639 with GFP
    Depositor
    Kevin White
    Insert
    ZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:179333542:G>T,rs978050614
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112345-plasmid-map-sequence-id-229659

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  15. pTBPL1-donor

    Plasmid
    #112316
    Purpose
    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP
    Depositor
    Kevin White
    Insert
    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs35776966, hg38:6:133987646:GTGTAT>-
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112316-plasmid-map-sequence-id-228480

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  16. PKN2 (PRKCL2A-c038)

    Plasmid
    #110265
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRKCL2 (PKN2 Human)
    Use
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110265-plasmid-map-sequence-id-212273

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  17. pcDNA3-Flag-Hsp40-S149A/S151A

    Plasmid
    #84998
    Purpose
    Hsp40/DnaJB1 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp40 (DNAJB1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 149 and 151 mutated to Alanine
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. KCTD17

    Plasmid
    #74661
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCTD17A (KCTD17 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. psicheck_NDUFA6_3UTR_mut

    Plasmid
    #65971
    Purpose
    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR, with two mutated tandem CAC motifs
    Depositor
    Thomas Tuschl
    Insert
    NDUFA6 (NDUFA6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated tandem CAC motif to CCC motif
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. 4GLM

    Plasmid
    #51313
    Depositor
    Cheryl Arrowsmith
    Insert
    4GLM (DNMBP Human, 4GLM)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2061 - 2080 of 48752 results