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Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES
Plasmid#69909PurposeExpresses a GFP circular RNA containing the sense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)
Plasmid#168191PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nLag6-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)
Plasmid#168097PurposeHA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.DepositorInsertHA-nGFP-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)
Plasmid#60606PurposeMammalian Display vectorDepositorInsertPS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
UseTagsC9ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047
Plasmid#60649PurposeExpresses a miniature version of the ZKSCAN1 circular RNADepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES
Plasmid#69910PurposeExpresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
Plasmid#73209PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX
Plasmid#202606PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-1P-P2A-mCherry-CAAX
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)
Plasmid#171375PurposeFor the mammalian expression of rat Gαi3 internally tagged with YFPDepositorInsertGai3-YFP
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)
Plasmid#69898PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in mammalian cellsDepositorUseTagsExpressionMammalianMutationF169L in ZKSCAN1PromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
(367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)
Plasmid#168099PurposeHA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nUbc-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag RdgBbeta (long form) splice variant 1
Plasmid#58275Purposemammalian expression of human RdgBbeta splice variant 1DepositorInsertRdgBbeta (PITPNC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-sfVenus-iGluSnFR
Plasmid#187097PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-sfVenus-iGluSnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR
Plasmid#187098PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-sfVenus-iGluSnFR
UseTagsiRFP670-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)
Plasmid#51369DepositorInsertDicer (DICER1 Human)
UseTagsFLAGExpressionMammalianMutation3' pocket mutant (Y926F, R927A)PromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)
Plasmid#49061PurposeExpresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and mVenusExpressionMammalianMutationS150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …PromoterCMVAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5065 pcDNA3.1/V5-His-TOPO hBrd4 (888-1362)
Plasmid#14444DepositorInsertBrd4 (888-1362) (BRD4 Human)
UseTagsExpressionMammalianMutationamino acids 888-1362PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)
Plasmid#69904PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in mammalian cellsDepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationF169L in ZKSCAN1PromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only