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Plasmid#122702PurposeDesigned for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only
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pSKAP_AO
Plasmid#122717PurposepSKAP plasmid containing the unique DNA barcode "AO" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.DepositorInsertBarcode AO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKAP_AI
Plasmid#122711PurposepSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.DepositorInsertBarcode AI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKAP_AH
Plasmid#122710PurposepSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.DepositorInsertBarcode AH
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKAP_AG
Plasmid#122709PurposepSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.DepositorInsertBarcode AG
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKAP_AE
Plasmid#122707PurposepSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identificationDepositorInsertBarcode AE
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsaGSPdR
Plasmid#108205PurposePdRDepositorInsertPutidaredoxin reductase
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHPro54G
Plasmid#108204Purpose4th Pro5DepositorInsertPro-Pro-Pro-Pro-Pro
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable sinceApril 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Level 2Δ
Plasmid#102708PurposeMobius Assembly Level 2 Acceptor Vector ΔDepositorInsertJ23106:B0034:sfGFP:rrnBT1-T7TE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Level 2B
Plasmid#102706PurposeMobius Assembly Level 2 Acceptor Vector BDepositorInsertJ23106:B0034:sfGFP:rrnBT1-T7TE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Level 2Γ
Plasmid#102707PurposeMobius Assembly Level 2 Acceptor Vector ΓDepositorInsertJ23106:B0034:sfGFP:rrnBT1-T7TE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::glgP_gap1
Plasmid#101845PurposeReplaces native promoter of glgP_gap1 with Ptrc promoterDepositorInsertCmR casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-fabI-gltA1-gltA2-udhA-zwf-gapAP1
Plasmid#87149PurposefabI-gltA1-gltA2-udhA-zwf-gapAP1 targeting guide RNA E. coli, Low Phosphate InductionDepositorInsertfabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-Maqu_3494
Plasmid#86205PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-PP_3456
Plasmid#86203PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertsEfflux pump
Efflux pump
UseTagsExpressionMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB8
Plasmid#82037PurposeHybrid CitA[1-193]-Tar[204-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB11
Plasmid#82040PurposeHybrid NarQ[1-217]-Tar[257-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-Maqu_0582
Plasmid#86204PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
UseTagsExpressionMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP
Plasmid#80912PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationPromoterP5G6T and PM,5G6TAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lasso 1
Plasmid#69935PurposeThe first of the two RNA lassos required for the RNA chain constructionDepositorInsertLasso 1
UseSynthetic BiologyTagsExpressionMutationPromoterTAATACGACTCACTATAGGGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only