Addgene

We narrowed to 3,036 results for: aaas

Showing: 2041 - 2060 of 3036 results

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PX458_BCL6_iso1_2

Plasmid

#104047

Purpose

Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 gRNA (BCL6 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

Plasmid

#178110

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

Plasmid

#32736

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Tags

Expression

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
AURKA A2.1 gRNA

Plasmid

#90541

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKA (Guide Designation A2.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB1-1

Plasmid

#121533

Purpose

sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB1-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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UBA1 G5.3 gRNA

Plasmid

#90932

Purpose

3rd generation lentiviral gRNA plasmid targeting human UBA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UBA1 (Guide Designation G5.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.CMV/CB-EGFP

Plasmid

#121508

Purpose

Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122022

Purpose

Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (AUU…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb4-GFP KI

Plasmid

#139663

Purpose

Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1-B

Plasmid

#44131

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

c-IAP1 (BIRC2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106355

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

MYOD1 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_sgGFP3

Plasmid

#119875

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pOPTXcGMPRELUC

Plasmid

#68503

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)

Use

Tags

luciferase

Expression

Bacterial and Plant

Mutation

3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

Promoter

OPTX promoter with cGMPRE

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
CBX3 A6.5 gRNA

Plasmid

#90569

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX3 (Guide Designation A6.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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PRC1 G2.2 gRNA

Plasmid

#90857

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PRC1 (Guide Designation G2.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSuper-PACSIN1-shRNA

Plasmid

#72576

Purpose

Expresses shRNA against PACSIN1

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

PACSIN1 (Pacsin1 Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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pSC50

Plasmid

#104824

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.08g081600, Glyma.05g126600

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo U6-SpAi9L-U6SpDMDL

Plasmid

#78608

Purpose

Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9L-U6SpDMDL

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pBW1997_pExpr-hU6-CRISPR-DECODER

Plasmid

#87549

Purpose

Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

gRNAs targeting NGN2, MIAT, ACTC1, TTN

Use

CRISPR, Cre/Lox, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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