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Plasmid#223166Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-smGFP-Kv2.1
Plasmid#131709PurposeExpresses Kv2.1 in mammalian cellsDepositorInsertKCNB1 (KCNB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-codon optimized catalytically inactive PHGDH
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
C76m
Plasmid#120988PurposeMoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertKanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD47_52
Plasmid#1446DepositorTypeEmpty backboneUseTagsSp6p, T7p, OriLam>>, OriLam<<, KanRN,…ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pHflu3
Plasmid#203881PurposepHI1 with insertion of kanamycin-resistance geneDepositorInsertaminoglycoside O-phosphotransferase APH(3')-IIa
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EH1
Bacterial Strain#102804PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.DepositorBacterial ResistanceNoneAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSG360
Plasmid#27155DepositorTypeEmpty backboneUseMycobacterial ko vectorTagsExpressionMutationPromoterAvailable sinceMarch 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSG360zeo
Plasmid#27154DepositorTypeEmpty backboneUseMycobacterial ko vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDB88
Plasmid#26958DepositorInserte. coli galactokinase
UseTagsExpressionBacterialMutationcloned under the MOP promoterPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1
Plasmid#32022DepositorInsertDCR1
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC
Plasmid#32023DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1(1-384)
Plasmid#32031DepositorInsertDCR1(1-384)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaNdeltaC
Plasmid#32030DepositorInsertDCR1(107-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1delta2d
Plasmid#32028DepositorInsertDCR1(15-260)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
RF17
Bacterial Strain#102801PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.DepositorBacterial ResistanceNoneAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
RF18
Bacterial Strain#102802PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.DepositorBacterial ResistanceNoneAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/v5-DEST hGlut2
Plasmid#18086DepositorInsertGlut2 (SLC2A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/v5-DEST hGlut4
Plasmid#18087DepositorInsertGlut4 (SLC2A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only