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Plasmid#210740PurposeCoding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4DepositorInsertsUseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationPromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only
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pUC57-ITR2-H1-GFP sgRNA Gollum Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210745PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 1DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 1
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210742PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 2
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2T_eGFP
Plasmid#208883PurposeExpress eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations L191T and A225TPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2E_eGFP
Plasmid#208884PurposeExpress eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations K192E and R233EPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro
Plasmid#210856PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationL324D/R543D/R548DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-L476/L479D
Plasmid#210845PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationL476/L479DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (450-517)-A452D/F453D
Plasmid#210851PurposeMammalian expression of ATG13 (450-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationA452D/F453DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-450)
Plasmid#210849PurposeMammalian expression of ATG13 (363-450) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-F509/F512D
Plasmid#210847PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationF509/F512DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-L495/L499D
Plasmid#210846PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationL495/L499DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-F466D/F470D
Plasmid#210844PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationF466D/F470DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-OH
Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
UseTagsollas and his-6 added to C terminus of protein co…ExpressionInsectMutationPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-kkv-3::NG
Plasmid#138960PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsert3' end of kkv and neon green (kkv Fly)
UseAn editing vector for drosophilaTagsNeon Green fused to C terminus of KkvExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-460)
Plasmid#203553PurposeMammalian expression of GST tagged ATG13 (363-460)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF CAG mc2 IRES neo
Plasmid#206232PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT58
Plasmid#204304PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (363-517)-MBP
Plasmid#203561PurposeMammalian expression of ATG13 (363-517) with MBP tagDepositorInsertATG13 (ATG13 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX
Plasmid#202719PurposeMammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imagingDepositorArticleInsertsMyr-Pink Flamindo
EGFP-bPAC-CAAX
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only