Addgene

We narrowed to 2,629 results for: GUS

Showing: 2021 - 2040 of 2629 results

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p3E-OSBP

Plasmid

#67677

Purpose

Multisite gateway entry clone for adding C-terminal fusions of OSBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

OSBP

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

aa 87-185 of NP_002547.1

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab8a

Plasmid

#109657

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab8a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab8a (rab8a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC

Plasmid

#100031

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC

Use

Tags

1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

Expression

Mammalian

Mutation

Promoter

Adenovirus major late promoter

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST15-Nedd4.2F823A

Plasmid

#165104

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d F823A

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

Insert

Nedd4.2d F823A (NEDD4L Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

F823A; mutation for destabilization of trimer fo…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb

Plasmid

#109579

Purpose

Multisite gateway vector for 3' tagging with RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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cpc-PHLS (g)

Plasmid

#52311

Purpose

Replaces the cpc operon in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Synechocystis endogenous cpc-operon promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 340-590

Plasmid

#11633

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 2.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
p3E-Dnm2b-DN

Plasmid

#109574

Purpose

Multisite gateway vector for 3' tagging with dominant negative Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

dominant negative

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-EHD1b-dEH

Plasmid

#109567

Purpose

Multisite gateway vector for 3' tagging with EHD1b-delta-EH

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD1b (ehd1b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

delta-EH

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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psbA2-Ptrc-T7-g10-PHLS (e)

Plasmid

#52310

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc-T7

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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psbA2-PHLS (b)

Plasmid

#52307

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

psbA2

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pJAF210

Plasmid

#52406

Purpose

Expresses Flag-tagged mouse Arf5

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf5 (Arf5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pJAF214

Plasmid

#52404

Purpose

Expresses Flag-tagged mouse Arf3

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf3 (Arf3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
psbA2-Ptrc-PHLS (d)

Plasmid

#52309

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 308-579

Plasmid

#11632

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal common domain of merlin.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pME-2PH-PLCd

Plasmid

#71283

Purpose

Multisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLC delta (Human)

Use

Gateway multisite middle entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

BsmBI

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-isoform a

Plasmid

#225447

Purpose

Express EGFP-fusion protein of FXR1 isoform a

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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