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Viral Prep#191737-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) (#191737). In addition to the viral particles, you will also receive purified AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) plasmid DNA. minBG-driven expression of SYFP2 in striatal direct pathway medium spiny neurons (D1-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsSYFP2Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only
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AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) (AAV PHP.eB)
Viral Prep#191707-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) (#191707). In addition to the viral particles, you will also receive purified AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) plasmid DNA. minBG-driven expression of SYFP2 in striatal indirect pathway medium spiny neurons (D2-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsSYFP2Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) (AAV PHP.eB)
Viral Prep#191726-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) (#191726). In addition to the viral particles, you will also receive purified AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) plasmid DNA. Expression of SYFP2 in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsNoneAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) (AAV PHP.eB)
Viral Prep#191728-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) (#191728). In addition to the viral particles, you will also receive purified AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) plasmid DNA. SYFP2 expression in striatal and neocortical Sst-Chodl neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsSYFP2Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) (AAV PHP.eB)
Viral Prep#214846-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) (#214846). In addition to the viral particles, you will also receive purified AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) plasmid DNA. minBG-driven expression of ChR2(H134R)-EYFP in striatal cholinergic neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsEYFPAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (AAV PHP.eB)
Viral Prep#214858-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (#214858). In addition to the viral particles, you will also receive purified AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) plasmid DNA. minBG-driven expression of SYFP2 plus a separate nuclear FLAG epitope in striatal cholinergic neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsSYFP2Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-sCAG-GluClβ.CreON
Plasmid#196996PurposeCre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClβ -YFP
UseAAV and Cre/LoxTagsYFPExpressionMutationPromotershort CAGAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJN601
Plasmid#59790Purposezf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a markerDepositorInsertsZF1 domain from pie-1
N-terminal half of yfp with introns
intron containing floxed unc-119 gene in reverse orientation
C-terminal half of yfp with introns
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJJB207
Plasmid#218596PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
UseTagsUbiY degradation tag and ePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKT001
Plasmid#218594PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
UseTagsUbiY degradation tag and ePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrans_9xD3B_Cluc_Norm
Plasmid#68415PurposeCRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.DepositorInsertCypridina Luciferase-2A-Venus YFP
UseLuciferaseTagsExpressionMammalianMutationYFP: M153T,V163A,S175G ("Venus") YFP Va…PromoterminimalCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_9xD3B_Cluc_Norm
Plasmid#68414PurposeCRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.DepositorInsertCypridina Luciferase-2A-Venus YFP
UseLentiviral and LuciferaseTagsExpressionMutationYFP: M153T,V163A,S175G ("Venus") YFP Va…PromoterminimalCMVAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAM5081
Plasmid#85107PurposePtrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.DepositorInsertYFP-KaiC
UseTagsYFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDH22
Plasmid#83798PurposeUseful for tagging yeast proteins with N-terminal YFP tag; G418 selectionDepositorInsertYFP
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIYneo
Plasmid#52117Purposea bicistronic IRES-YFP containing vector for mammalian expression (CMV promoter)DepositorTypeEmpty backboneUseTagsIRES-YFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM5087
Plasmid#85123PurposePtrc-YFP subcloned from pAM5080 into NSII-Km vector.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDH6
Plasmid#83777PurposeUseful for tagging yeast proteins with C-terminal YFP tag; G418 selectionDepositorInsertYFP
UseTagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y mutations added.PromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0068
Plasmid#196179PurposeYFP expression cassette: CaMV35S::TMV::YFP::35sTDepositorInsertCaMV35S::TMV::YFP::35sT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only