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Addgene
Showing: 2001 - 2020 of 48752 results
  1. SOX17-tdTomato-EhB

    Plasmid
    #210467
    Purpose
    Donor plasmid for knock-in tdTomato into the human SOX17 locus
    Depositor
    Insert
    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pLV CMV FST-FLAG

    Plasmid
    #175167
    Purpose
    Lentiviral expression of human FST-FLAG
    Depositor
    Insert
    FST (FST Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pRK GFP Paxillin

    Plasmid
    #50529
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Insert
    Paxillin (PXN Human)
    Use
    Tags
    GFP21
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. 3XMEF2-luc

    Plasmid
    #32967
    Purpose
    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter
    Depositor
    Insert
    MEF2 binding sites (MEF2A Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    MEF2
    Available since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
  6. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pENTR223 CD147, open

    Plasmid
    #149720
    Purpose
    for Gateway cloning of CD147 into destination vector
    Insert
    CD147 (BSG Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    wt, without stop codon
    Promoter
    Available since
    June 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pAAV hSyn-Flex RG-cerulean

    Plasmid
    #98221
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
    Depositor
    Insert
    Cerulean-E2A-RG
    Use
    AAV
    Tags
    cerulean
    Expression
    Mutation
    Promoter
    human Synapsin
    Available since
    Aug. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51083
    Purpose
    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Has Service
    AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3.2-V5-DEST-hSFRP1-his

    Plasmid
    #189540
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    his
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA3.2-V5-DEST-hSFRP1-VF

    Plasmid
    #189542
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pWPI-IRES-Puro-Ak-ACE2

    Plasmid
    #154985
    Purpose
    Lentiviral vector expressing Human ACE2
    Depositor
    Has Service
    Lentiviral Prep
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. T-IL2-12

    Plasmid
    #166564
    Purpose
    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pLPS-hSox2-gfp

    Plasmid
    #49390
    Purpose
    mammalian expression of human Sox2 fused to EGFP
    Depositor
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
  16. Q-IL18-6

    Plasmid
    #166554
    Purpose
    scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pWPI-IRES-Bla-Ak-ACE2

    Plasmid
    #154981
    Purpose
    Lentiviral vector expressing Human ACE2
    Depositor
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 2001 - 2020 of 48752 results