Addgene

We narrowed to 4,007 results for: gast

Showing: 1981 - 2000 of 4007 results

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pAc5.1B-EGFP-DmDCP1_327-372_H

Plasmid

#146500

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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1056D

Plasmid

#183132

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056E

Plasmid

#183133

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[dsx]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056F

Plasmid

#183134

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[tra]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF_340-368-GST-V5His6_AH

Plasmid

#148935

Purpose

Insect Expression of DmGIGYF_340-368

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_340-368 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

Plasmid

#147580

Purpose

Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PC(1-86)-H6

Plasmid

#183780

Purpose

Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Use

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAct-GST-H3(K27Q)

Plasmid

#182843

Purpose

a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

GST-histone H3 fusion

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Actin5C

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAct-GST-H3

Plasmid

#182842

Purpose

A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

GST-histone H3 fusion

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Actin5C

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6.3

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-DmRoquin_AD

Plasmid

#148531

Purpose

Mammalian Expression of DmRoquin

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin (roq Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1200-1384_L

Plasmid

#146825

Purpose

Insect Expression of DmGW182_1200-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_1200-1384 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmPAN3_349-790-P652G_T

Plasmid

#147570

Purpose

Bacterial Expression of DmPAN3_349-790-P652G

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-P652G (CG11486 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G

Plasmid

#146396

Purpose

Insect Expression of DmGW182-delUBAdelQrich

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmGW182-delUBAdelQrich (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_57-499_B

Plasmid

#145979

Purpose

Insect Expression of DmHPat_57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-499 (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-510-V5His6_D

Plasmid

#146151

Purpose

Insect Expression of Dm4E-T_1-510

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510 (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B_1-267_D

Plasmid

#146118

Purpose

Insect Expression of DmMe31B_1-267

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B_1-267 (me31B Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D

Plasmid

#146123

Purpose

Insect Expression of DmEDC3_1-440

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-440 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D

Plasmid

#146149

Purpose

Insect Expression of Dm4E-T_1-510

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510 (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-DFDF-KFKF_C

Plasmid

#146036

Purpose

Insect Expression of DmEDC3-DFDF-KFKF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-DFDF-KFKF (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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