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Plasmid#222414PurposeTo express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertBAF (BANF1 Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-pA
Plasmid#220752PurposeExpression of Sun1GFP for nuclei isolationDepositorInsertSun1-GFP-WPRE
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-GST-ATG13 (363-517)
Plasmid#222823PurposeBacteria expression of His-GST -ATG13 C-terminalDepositorInsertATG13 (ATG13 Human)
UseTags6His-GSTExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18-LucFF
Plasmid#220496PurposeTo generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX-SFFV_NKX2-1_Short_V5-GFP
Plasmid#221500PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_CdTag-HA
Plasmid#221493PurposeNKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_W208L
Plasmid#221486PurposeNKX2-1 W208L DNA binding mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationW208LPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-UBC_NKX2-1
Plasmid#221495PurposeNKX2-1 short isoform lentiviral overexpression using UBC promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterUBCAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelCTerm_1-223AA
Plasmid#221476PurposeGateway cloning of NKX2-1 C-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 224-371AA C-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelNTerm_160-371AA
Plasmid#221475PurposeGateway cloning of NKX2-1 N-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelNTerm_160-371AA
Plasmid#221487PurposeNKX2-1 N-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_V205F
Plasmid#221485PurposeNKX2-1 V205F DNA binding mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationV205FPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-hPGK_NKX2-1_Short_V5-GFP
Plasmid#221498PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterhPGKAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelCTerm_1-223AA
Plasmid#221488PurposeNKX2-1 C-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 224-371AA C-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-hPGK_NKX2-1
Plasmid#221494PurposeNKX2-1 short isoform lentiviral overexpression using hPGK promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterhPGKAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-SFFV_NKX2-1
Plasmid#221496PurposeNKX2-1 short isoform lentiviral overexpression using SFFV promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-UBC_NKX2-1_Short_V5-GFP
Plasmid#221499PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterUBCAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_Short_V5-GFP
Plasmid#221497PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only