Showing: 1981 - 2000 of 14062 results
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Plasmid#122674PurposeExpresses mouse PCSK3 in mammalian cellsDepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
UseTagsExpressionMammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR-NLS (EIF4EBP1 Synthetic, Human)
UseTagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R3
Plasmid#61384PurposeExpresses TRAIL receptor 3DepositorInsertTRAIL receptor 3 (TNFRSF10C Human)
UseTagsHA tag and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R3 leader peptide (aa1-29) replaced with TN…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R4
Plasmid#61385PurposeExpresses HA tagged TRAIL receptor 4DepositorInsertTRAIL receptor 4 (TNFRSF10D Human)
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R4 leader peptide (aa1-29) replaced with TN…PromoterAvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)AvailabilityAcademic Institutions and Nonprofits only -
TfR-moxGFP-DHFR
Plasmid#173012PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).DepositorInsertTfR-moxGFP-DHFR
UseTagsDHFR and moxGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag
Plasmid#154970PurposeExpresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.DepositorInsertPfEBA175
UseTagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationinsert codes for EBA175 residues 1284 to 1462PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR
Plasmid#164569PurposeSARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)DepositorInsertSpike (S-RRAR variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mClover3-Puromycin
Plasmid#167205PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsmClover3ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mTagBFP2-Blasticidin
Plasmid#167207PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with BlasticidinDepositorTypeEmpty backboneUseCRISPRTagsmTagBFP2ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-NanoLuc-Puromycin
Plasmid#167209PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsNanoLucExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybSEP2-WPRE
Plasmid#207661PurposeMonitoring neuropeptide releaseDepositorInsertCytochrome b-561 (Cyb561 Mouse)
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted 2 X SE…PromoterhSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
UseTagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailabilityAcademic Institutions and Nonprofits only
Showing: 1981 - 2000 of 14062 results