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Plasmid#86780PurposeExpresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.DepositorInsertLN*
UseUstilago maydis expressionTagsGfpExpressionBacterialMutationPromoterPotefAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(272)
Plasmid#44376DepositorInsertUAS1B8-TEF(272) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(272)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT5-TEF2p-ycrtYB-ADH1t
Plasmid#65359PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-TEF2p-ycrtE-ADH1t
Plasmid#65353PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(1004)
Plasmid#44365DepositorInsertUAS1B16-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSM-ab-Psptef1-NAT-Ttip1
Plasmid#127747PurposeYeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.DepositorInsertnourseothricin N-acetyltransferase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptef1Available sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(1004)
Plasmid#44366DepositorInsertUAS1B8-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
POT4-TEF2p-ycrtI-ADH1t
Plasmid#65356PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTEF2p-ycrtI-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(203)
Plasmid#44378DepositorInsertUAS1B8-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(203)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B8-TEF(804)
Plasmid#44368DepositorInsertUAS1B8-TEF(804) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(804)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPpT4- TEF2prom-TIR1-FLAG
Plasmid#189726PurposeEnables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterTEF2Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG
Plasmid#139963PurposeOver-expression of native 3-hydroxy-3-methyl glutaryl CoA reductaseDepositorInsertConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG
Plasmid#139964PurposeOver-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductaseDepositorInsertConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5P-ECM33_intron
Plasmid#127599Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-ECM33_intron
Plasmid#127598Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-no_intron
Plasmid#127597Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoterDepositorInsert1xFLAG-MCP expression cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only