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Showing: 181 - 200 of 50464 results

  1. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid
    #101208
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A and Q695A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101208-plasmid-map-sequence-id-198110

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  2. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.
    Depositor
    Aindrila Mukhopadhyay
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. Plasmid-ID Kit

    Plasmid Kit
    #1000000168
    Purpose
    The Plasmid-ID Kit allows for the high-throughput assessment of competitive and effective rhizobial strains.
    Depositor
    Philip Poole
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only

  4. Integrated Genomics Plasmids

    Plasmid Kit
    #1000000004
    Purpose
    Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable
    Depositor
    Guy Caldwell
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. NT-CRISPR plasmid collection

    Plasmid Kit
    #1000000189
    Purpose
    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.
    Depositor
    Anke Becker
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  6. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.
    Depositor
    Stephan Gruber
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  7. MultiSite Gateway plasmid toolKit

    Plasmid Kit
    #1000000050
    Purpose
    Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules
    Depositor
    Tomas Santalucia
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. Hamdoun lab FP fusion plasmids

    Plasmid Kit
    #1000000033
    Purpose
    Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)
    Depositor
    Amro Hamdoun
    Available since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. T-B18

    Bacterial Strain
    #159446
    Purpose
    E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon
    Depositor
    Maciek R. Antoniewicz, Eleftherios Terry Papoutsakis
    Article
    Har et al Appl Microbiol Biotechnol. 2021 Jan;105(
    Bacterial Resistance
    Ampicillin and kanamycin
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. UQ5827

    Bacterial Strain
    #37174
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. UQ5875

    Bacterial Strain
    #37167
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin and kanamycin
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  15. UQ5457

    Bacterial Strain
    #37165
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin
    Species
    Burkholderia xenovorans
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. UQ5871

    Bacterial Strain
    #37170
    Depositor
    Gary Roberts
    Bacterial Resistance
    Kanamycin
    Available since
    Aug. 23, 2012
    Availability
    Academic Institutions and Nonprofits only

  17. UQ6106

    Bacterial Strain
    #37176
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  18. UQ5828

    Bacterial Strain
    #37175
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  19. UQ5784

    Bacterial Strain
    #37173
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  20. UQ5874

    Bacterial Strain
    #37169
    Depositor
    Gary Roberts
    Bacterial Resistance
    Ampicillin and kanamycin
    Available since
    Aug. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 50464 results