Skip to main content
Addgene
Showing: 181 - 200 of 592 results

  3. pCS2+ H2B-mRuby2

    Plasmid
    #99270
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mRuby2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    mRuby2-3XHA
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    99270-plasmid-map-sequence-id-192969

    Enlarge

  4. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid
    #106204
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106204-plasmid-map-sequence-id-204613

    Enlarge

  6. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid
    #196677
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIα-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196677-plasmid-map-sequence-id-385124

    Enlarge

  7. pKK-mRuby3-TEV

    Plasmid
    #105780
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mRuby3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105780-plasmid-map-sequence-id-203369

    Enlarge

  8. pAG416GAL-ccdB-mRuby3

    Plasmid
    #166139
    Purpose
    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Type
    Empty backbone
    Use
    Tags
    mRuby3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166139-plasmid-map-sequence-id-328575

    Enlarge

  9. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

    Plasmid
    #106205
    Purpose
    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184V + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106205-plasmid-map-sequence-id-204612

    Enlarge

  13. C1-MPAct(delta1-6)-mRuby3

    Plasmid
    #155223
    Purpose
    F-actin bidning mutant of MPAct
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Deletion of first 6 AA from F-tractin (9-14 from …
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155223-plasmid-map-sequence-id-310127

    Enlarge

  17. pKK-TEV-mRuby3

    Plasmid
    #105797
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105797-plasmid-map-sequence-id-203388

    Enlarge

  18. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid
    #106206
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A + mRuby3 fusion
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106206-plasmid-map-sequence-id-201033

    Enlarge

  20. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid
    #196678
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIαmut-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196678-plasmid-map-sequence-id-385123

    Enlarge

Showing: 181 - 200 of 592 results