1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pSGE_881_Nhe2_EcR-to-HLH4C

   Plasmid

   #71186

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   881_Nhe2_EcR-to-HLH4C
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   DSCP Drosophila synthetic core promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor

   Plasmid

   #211866

   Purpose

   CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   CENP-T(1-242)-I3-01 (CENPT Synthetic, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor

   Plasmid

   #211867

   Purpose

   CENP-T(1-242) transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   CENP-T(1-242) (CENPT Synthetic, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

   Plasmid

   #211861

   Purpose

   Nuclear-localized E3-DART ligase in domestication vector.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Promoter

   Available since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

   Plasmid

   #211863

   Purpose

   Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA

   Expression

   Mutation

   C492A

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMB-LEr

   Plasmid

   #61186

   Purpose

   Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtRAB5A2
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

   Plasmid

   #201186

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 1 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK375 (Hygro-P2A-Stag-3FLAG)

   Plasmid

   #121186

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-Stag-3FLAG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOVC1_X_CrPH-LOV

    Plasmid

    #131186

    Purpose

    Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    CrPH-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid

    #81186

    Purpose

    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    

    Use

    CRISPR

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcdna3.1_mouseST6galI

    Plasmid

    #178435

    Purpose

    murine St6gal1 expression plasmid

    Depositor

    Deborah Fass

    Article

    Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

    Insert

    St6gal1 (St6gal1 Mouse)
    

    Use

    Tags

    6xHIS-TEV Cleavage Site

    Expression

    Mammalian

    Mutation

    Coding sequnce for amino acids 85-403

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid

    #111862

    Purpose

    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDN(1-400)-GFP (Bicd2 Mouse)
    

    Use

    Tags

    8xHis tag, GFP, and ZZ tag

    Expression

    Insect

    Mutation

    Codon optimized for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hook3(1-522)-SNAPf-Psc-StrepII

    Plasmid

    #111860

    Purpose

    C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    Hook3(1-552)-SNAPf (HOOK3 Human)
    

    Use

    Tags

    Psc, SNAPf, and Strep-II

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hook3(1-522)-GFP-Psc-StrepII

    Plasmid

    #111861

    Purpose

    C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    Hook3(1-552)-GFP (HOOK3 Human)
    

    Use

    Tags

    GFP, Psc tag, and Strep tag

    Expression

    Insect

    Mutation

    Optimised for expression in SF9 cells

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SLC26A3_STOP

    Plasmid

    #161186

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC26A3 (SLC26A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pBiT3.1-C_CIAO1:1-339

    Plasmid

    #211186

    Purpose

    Mammalian expression of full-length human CIAO1. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    CIAO1:M1-L339 (CIAO1 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. rk16s

    Plasmid

    #117436

    Purpose

    Expression of ARL4

    Depositor

    Richard Kahn

    Insert

    ARL4 (ARL4A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rk4s

    Plasmid

    #117434

    Purpose

    Expression of ARL4a

    Depositor

    Richard Kahn

    Insert

    ARL4a (ARL4A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge