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Plasmid#11007DepositorInsertTRP1 (TRP1 Budding Yeast)
UseTagsV5 and his6ExpressionBacterial and YeastMutationORF-TRP1 without stop codonPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pBTK569
Plasmid#110614PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoterDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationV32M (see comments below)PromoterAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.3-OGDH_wobble
Plasmid#110938PurposeOGDH ORF containing silent wobble mutations insensitive to corresponding shRNAsDepositorInsertOGDH wobble cDNA (OGDH Human)
UseLentiviralTagsExpressionMutation15 silent wobble mutations corresponding to three…PromoterSV40Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK229
Plasmid#110591PurposeBTK Type 3 coding sequence for golden gate assembly and homologous recombinationDepositorInsertKanamycin resistance
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-FNLS-PGK-Puro
Plasmid#110844PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-4A-VN
Plasmid#110647Purposeexpresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR bindingDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsDDK and VNExpressionMammalianMutationC379A, C385A, C421A and C427APromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN-2xFKBP-mEGFP-NDP52
Plasmid#110465Purposeretrovirus construct for stable expressing mEGFP tagged NDP52DepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviral; RetrovirusTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK527a
Plasmid#110606PurposeBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p079 pPNT6
Plasmid#11072DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shRFP
Plasmid#110940PurposeTet-inducible shRNA targeting RFPDepositorInsertshRFP
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK501
Plasmid#110602PurposeBTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)DepositorInsertGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK570
Plasmid#110615PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoterDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
hOC-GFPtpz
Plasmid#110207PurposeExpresses GFP in mature bone cells.DepositorInserteGFPtopaz
UseTagsNoneExpressionMammalianMutationPromoterHuman osteocalcin (BGLAP)Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-TdTomato
Plasmid#110338PurposeTdTomato reporter vector of iTango and Cal-Light system for AAV productionDepositorInserttetO-TdTomato
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RAPGEF2
Plasmid#110162PurposeExpression of human RAPGEF2 in mammalian cellsDepositorInsertRAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-dLUC
Plasmid#110056PurposeCry1 transcription luciferase reporterDepositorInsertCry1 promoter and luciferase (Cry1 Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCry1Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 WT
Plasmid#110125PurposeExpresses Flag-tagged human UAP56 (DDX39B)DepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only