Addgene

We narrowed to 5,454 results for: #16

Showing: 181 - 200 of 5454 results

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) TFRC

Plasmid

#74709

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

Plasmid

#83489

Purpose

cre-dependent PAK1-AID under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

PAK1-AID-ires-EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

Plasmid

#83490

Purpose

cre-dependent CA-AMPK under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

CA-AMPK-2A-mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

H150R

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

Plasmid

#83491

Purpose

cre-dependent DN-AMPK under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

DN-AMPK-2A-mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

K45R

Promoter

Availability

Academic Institutions and Nonprofits only

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pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2

Plasmid

#60388

Purpose

Expression of yeast beta tubulin (TUB2) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL

Availability

Academic Institutions and Nonprofits only
pKP1626

Plasmid

#167646

Purpose

Express FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

fepA D34C, delta12-16 (TonB box)

Use

Tags

Expression

Bacterial

Mutation

fepA with D34C, delta12-16 (TonB box) mutations. …

Promoter

Availability

Academic Institutions and Nonprofits only

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pKP1627

Plasmid

#167648

Purpose

Express FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

fepA G54C, delta12-16 (TonB box)

Use

Tags

Expression

Bacterial

Mutation

fepA with G54C, delta12-16 (TonB box) mutations (…

Promoter

Availability

Academic Institutions and Nonprofits only

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pSPIKE-P

Plasmid

#101172

Purpose

chimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomics

Depositor

Philip Poole

Insert

V4 16S rRNA chimeric spike

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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FusX TALEN assembly system

Plasmid Kit

#1000000063

Purpose

Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics

Depositor

Stephen Ekker

Availability

Academic Institutions and Nonprofits only
pMB1610_pRR-Puro

Plasmid

#65853

Purpose

Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split puromycin N-acetyltransferase coding sequence

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1

Plasmid

#60385

Purpose

Expression of yeast alpha tubulin (TUB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2-Cterminal6xHis

Plasmid

#60386

Purpose

Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

6xHIS

Expression

Yeast

Mutation

Promoter

GAL

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 (1-428)

Plasmid

#60395

Purpose

Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

deleted 429 - end

Promoter

GAL

Availability

Academic Institutions and Nonprofits only
pRS424-TUB1-Cterminal6xHis

Plasmid

#60387

Purpose

Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

6xHIS

Expression

Yeast

Mutation

Promoter

GAL

Availability

Academic Institutions and Nonprofits only
pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Availability

Academic Institutions and Nonprofits only
pB-actin E6 E7

Plasmid

#13712

Depositor

Karl Munger

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV 16 E6 E7 (E6, E7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pED7x26

Plasmid

#134813

Purpose

Stringent phage propagation reporter containing pIII-16-29-34-TAGA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

pIII 16-TAGA 29-TAGA 34-TAGA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

16-TAGA 29-TAGA 34-TAGA

Promoter

Availability

Academic Institutions and Nonprofits only

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