Addgene

We narrowed to 18,756 results for: LEA

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Human Lentiviral sgRNA Library - Nuclear

Pooled Library

#51047

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
CHyMErA LbCas12a Nuclease Optimization hgRNA Library

Pooled Library

#209734

Purpose

CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different LbCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only
CHyMErA AsCas12a Nuclease Optimization hgRNA Library

Pooled Library

#209735

Purpose

CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different AsCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only
Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-hHSF1 WT

Plasmid

#71724

Purpose

plasmid expressing myc-his tag hHSF1 WT

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

HSF1 (HSF1 Human)

Use

Tags

MYC 6xHIS

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HSF1Δ∼PRD

Plasmid

#71725

Purpose

plasmid expressing mic-his tag phosphorylation-deficient hHSF1

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

phosphorylation-deficient HSF1 (HSF1 Human)

Use

Tags

mac 6xHIS

Expression

Mammalian

Mutation

15 phosphorylation sites in RD mutated to Alanine

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1Δ∼PRD

Plasmid

#71727

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (Cerulean-Venus)

Plasmid

#18681

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (H6-mCerulean)

Plasmid

#29726

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - TEV and mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (u-mCerulean)

Plasmid

#29773

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET MBP mCerulean LIC cloning vector (MBP-mCerulean)

Plasmid

#29751

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - MBP -TEV and mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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mCerulean N1

Plasmid

#27795

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mCerulean N1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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mCerulean3-N1

Plasmid

#54730

Purpose

Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C1

Plasmid

#54605

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean C1

Plasmid

#27796

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mCerulean C1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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moxCerulean3

Plasmid

#68066

Purpose

C-terminal fusion to moxCerulean3

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Use

Tags

moxCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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Cerulean

Plasmid

#15214

Depositor

Dave Piston

Article

Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

Insert

monomeric Cerulean

Use

Tags

Expression

Mammalian

Mutation

A206K

Promoter

Available since

June 22, 2007

Availability

Academic Institutions and Nonprofits only

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pCerulean

Plasmid

#24756

Depositor

Martin Fraunholz

Article

Paprotka et al J Microbiol Methods. 2010 Aug 10. ():.

Insert

Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
Cerulean-N1

Plasmid

#54742

Purpose

Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson, Dave Piston

Article

Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

Type

Empty backbone

Use

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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