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Plasmid#186421PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2DepositorInsertdCas9-scFv fusion (anti-SARS-CoV-2 spike)
UseCRISPRTags6HisExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to nanobody (anti-beta catenin)
Plasmid#186420PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tagDepositorInsertdCas9-nanobody fusion (anti-beta catenin)
UseCRISPRTags6HisExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_H1_Gal4VP64
Plasmid#169918PurposeExpression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.DepositorInsertantiCD19-Notch1-GL4VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_Z3_Gal4VP64
Plasmid#169916PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhU6 and hUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Jun lab tunable CRISPRi strain
Bacterial Strain#86400PurposeStrain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-termDepositorBacterial ResistanceSpectinomycin and hygromycinAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJET-41-DExCon-antiGFPnanobody-mCherry-R11b
Plasmid#179901PurposeDonor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL-gRNA(anti-cGAS)-Cas9-Puro
Plasmid#130908PurposegRNA targeting human cGAS; Cas9 insert; Puromycin selection markerDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET-42-DExCon-antiGFPnanobody-mCherry-R11a
Plasmid#179902PurposeDonor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF8_isoD_CRISPR_resistant
Plasmid#122468PurposepDONR for Gateway cloningDepositorInsertSCAF8 isoform D CRISPR resistant (SCAF8 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF4_CRISPR resistant
Plasmid#122465PurposepDONR for Gateway cloningDepositorInsertSCAF4 CRISPR resistant (SCAF4 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF8_isoC_CRISPR resistant
Plasmid#122466PurposepDONR for Gateway cloningDepositorInsertSCAF8 isoform C CRISPR resistant (SCAF8 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/GFP-SCAF8 (CRISPR_resistant)
Plasmid#122472PurposeDox-inducible GFP (N-terminal tag) SCAF8 expressionDepositorInsertSCAF8 (SCAF8 Human)
UseTagsGFPExpressionMammalianMutationSynonymous mutations conferring resistance to gRN…PromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
Plasmid#122471PurposeDox-inducible GFP (N-terminal tag) SCAF4 expressionDepositorInsertSCAF4 (SCAF4 Human)
UseTagsGFPExpressionMammalianMutationSynonymous mutations conferring resistance to gRN…PromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro hGSDME gRNA2
Plasmid#223520PurposeKnocking out hGSDME in human cellsDepositorInsertGSDME (GSDME Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro hGSDME gRNA1
Plasmid#223519Purposeknocking out hGSDME in human cellsDepositorInsertGSDME (GSDME Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro mGSDME gRNA2
Plasmid#223522PurposeKnocking out mGSDME in mouse cellsDepositorInsertGSDME (GSDME Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro mGSDME gRNA1
Plasmid#223521PurposeKnocking out mGSDME in mouse cellsDepositorInsertGSDME (GSDME Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PL-CRISPR.EFS.tRFP-gIfngr1-1
Plasmid#166479PurposeExpresses spCas9, tRFP and a gRNA targeting mouse Ifngr1DepositorInsertgRNA for mouse Ifngr1 (Ifngr1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only