1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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1. pGBW-m4252961

   Plasmid

   #153590

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;TRX

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. lentiCRISPR.v2.TetON_Cas13d_U6_DR

   Plasmid

   #196727

   Purpose

   Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPRv2_PROX1_1+4

   Plasmid

   #121177

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

   Plasmid

   #118156

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118157

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118159

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118160

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiCRISPRv2R

    Plasmid

    #167932

    Purpose

    lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p8389 LentiCRISPRv2 Neo sgNT-1

    Plasmid

    #221649

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiGuide-TagBFP-2A-BSD

    Plasmid

    #167929

    Purpose

    Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GESTALT_pX330-v1

    Plasmid

    #103061

    Purpose

    plasmid px330 with guide targeting GESTALT v1 through V5 constructs

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p8390 LentiCRISPRv2 Neo sgNT-2

    Plasmid

    #221650

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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