175,559 results
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Plasmid#12259PurposeVSV-G envelope expressing plasmidDepositorInsertVSV G
UseLentiviral; EnvelopeExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
psPAX2
Plasmid#12260Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)DepositorTypeEmpty backboneUseLentiviral; PackagingExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianPromoterhU6Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV9)
Viral Prep#44362-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV.TBG.PI.Cre.rBG (AAV8)
Viral Prep#107787-AAV8PurposeReady-to-use AAV8 particles produced from AAV.TBG.PI.Cre.rBG (#107787). In addition to the viral particles, you will also receive purified AAV.TBG.PI.Cre.rBG plasmid DNA. TBG-driven expression of Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTBGAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV.rTH.PI.Cre.SV40 (AAV9)
Viral Prep#107788-AAV9PurposeReady-to-use AAV9 particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterrTHAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax
Plasmid#174820PurposeMammalian expression of SpCas9 PEmax prime editorDepositorInsertPEmax
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
ExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXWB-EBNA1
Plasmid#37624PurposeNon-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmidsDepositorAvailable SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP
Plasmid#13031PurposeMammalian expression vector for cloning your gene fused to EGFPDepositorInsertEnhanced Green Fluorescent Protein
ExpressionMammalianAvailable SinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry (AAV9)
Viral Prep#50459-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)
Viral Prep#52925-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)
Viral Prep#154867-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro
Plasmid#85132Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralPromoterEF1aAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV9)
Viral Prep#162377-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-tdTomato (AAV5)
Viral Prep#44332-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1 gfaABC1D-tdTomato (#44332). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-tdTomato plasmid DNA. GfaABC1D-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorTagstdTomatoAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only