168,810 results

1. pGP-AAV-syn-jGCaMP8m-WPRE (AAV PHP.eB)

   Viral Prep

   #162375-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

2. pMD2.G

   Plasmid

   #12259

   Purpose

   VSV-G envelope expressing plasmid

   Depositor

   Didier Trono

   Insert

   VSV G
   

   Use

   Lentiviral; Envelope

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. psPAX2

   Plasmid

   #12260

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral; Packaging

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV.TBG.PI.Cre.rBG (AAV8)

   Viral Prep

   #107787-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from AAV.TBG.PI.Cre.rBG (#107787). In addition to the viral particles, you will also receive purified AAV.TBG.PI.Cre.rBG plasmid DNA. TBG-driven expression of Cre. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   TBG

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pRSV-Rev

   Plasmid

   #12253

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   Rev (rev )
   

   Use

   Lentiviral; Packaging

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDLg/pRRE

   Plasmid

   #12251

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   HIV-1 GAG/POL
   

   Use

   Lentiviral; Packaging

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCmini-iCAP-PHP.eB

   Plasmid

   #103005

   Purpose

   non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   Synthetic construct isolate AAV-PHP.eB VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)

    Viral Prep

    #44361-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-hSyn-GRAB_ACh3.0 (AAV9)

    Viral Prep

    #121922-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_ACh3.0 (#121922). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_ACh3.0 plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Promoter

    Syn

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

12. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PcDNA3.1-Flag

    Plasmid

    #210342

    Purpose

    Empty Vector for molecular cloning

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    Flag tag
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (AAV8)

    Viral Prep

    #61592-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (#61592). In addition to the viral particles, you will also receive purified pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA plasmid DNA. CMV-driven expression of SaCas9. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

15. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral Prep

    #50459-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV1)

    Viral Prep

    #105553-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    Syn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2/5

    Plasmid

    #232922

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Sept. 17, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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