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Mouse Subpooled CRISPRa-v2 Libraries
(Pooled Libraries #83998, #83999, #84000, #84001, #84002, #84003, #84004)

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  • Purpose

    Version 2 - Mouse subpooled CRISPRa libraries for activation of gene transcription. Available as five gRNAs per gene.

  • Vector Backbone

    pCRISPRia-v2 (Plasmid #84832) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 83998

  • Kinases, Phosphatases, and Drug Targets †

1 $430 Add to Cart
Pooled Library 83999

  • Cancer and Apoptosis †

1 $325 Add to Cart
Pooled Library 84000

  • Stress and Proteostasis †

1 $325 Add to Cart
Pooled Library 84001

  • Mitochondria, Trafficking, and Motility †

1 $325 Add to Cart
Pooled Library 84002

  • Gene Expression †

1 $430 Add to Cart
Pooled Library 84003

  • Membrane Proteins †

1 $430 Add to Cart
Pooled Library 84004

  • Unassigned †

1 $325 Add to Cart
Available to academic and nonprofits only.

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with SunTag-VP64 constructs (#60903 and #60904) or otherwise used with cell lines already expressing activator dCas9.

  • If you would like to receive all seven sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted Targeting gRNAs Control gRNAs
83998 2,268 12,090 250
83999 2,856 15,195 280
84000 2,796 14,660 290
84001 2,099 11,040 250
84002 1,915 10,080 250
84003 2,104 10,975 250
84004 5,901 30,895 600
  • Species
    Mouse
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    12.5 ng/µL

Resource Information

Depositor Comments

In addition to SunTag-VP64 constructs (#60903 and #60904), another dCas9-containing construct that can be used with these libraries is dCas9-VPR (#63798).

The data analysis pipeline for analyzing sequencing data is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83998)
    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83999)
    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #84000)
    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #84001)
    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #84002)
    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #84003)
    mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #84004)

  • For your References section:

    Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255
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