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Qi Mouse Transcription Factor Activation Library
(Pooled Library #122131)

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  • Purpose

    This CRISPR activation library contains gRNAs targeting all mouse putative transcription factors according to the TRANSFAC database. This library activates target genes when used in combination with dCas9 activation systems, such as the SunTag system and the dCas9-VP64 system.

  • Vector Backbone

    pCRISPRia-v2 (Plasmid #84832) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 122131 mTF CRISPRa† 1
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with SunTag-VP64 constructs (#118592, #122132, and #122133) or otherwise used with cell lines already expressing activator dCas9.

Library Details

  • Species
    Mouse
  • Targets
    2,428 (including all isoforms)
  • gRNAs
    51,762
  • Controls
    9,296 non-targeting
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

In addition to the SunTag-VP64 constructs (#118592, #122132, and #122133), another dCas9-containing construct that could be used with this library is dCas9-VPR (#63798).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Transcription Factor Activation library was a gift from Stanley Qi (Addgene #122131)

  • For your References section:

    CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS. Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003. PubMed 30318302
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