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mTF-CRISPRai Library
(Pooled Library #122131)

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  • Purpose

    This CRISPRai library contains gRNAs targeting all mouse putative transcription factors according to the TRANSFAC database. The sgRNA design region spans -3000 bp to +1500 bp around the TSS of the target genes. This library activates target genes when used with dCas9 activation systems, such as the SunTag system and the dCas9-VP64 system. Conversely, it represses target genes when used with dCas9 repression systems, such as the dCas9-KRAB system.

  • Vector Backbone

    pCRISPRia-v2 (Plasmid #84832) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 122131 mTF CRISPRai† 1 $640 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with SunTag-VP64 constructs (#118592, #122132, and #122133) or dCas9-VPR (#63798) for activation of target genes; or with dCas9-KRAB (#112195, #110820, or #110821) for repression of target genes.

Library Details

  • Species
    Mouse
  • Targets
    2,428 (including all isoforms)
  • gRNAs
    93,107
  • Controls
    9,296 non-targeting
  • Lentiviral Generation
    2nd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTF-CRISPRai library was a gift from Stanley Qi (Addgene #122131 ; http://n2t.net/addgene:122131 ; RRID:Addgene_122131)

  • For your References section:

    CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS. Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003. PubMed 30318302
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