Mouse Liver CRISPR Knockout Library
(Pooled Library #192824)

• Purpose:

  This library targets 13,189 of the 13,266 protein-coding genes expressed in the mouse liver at any time point in development, adulthood, and regeneration (as determined by RNAseq, FPKM > 0.3), alongside 6,500 control sgRNAs. This includes an average of 5 sgRNAs per gene, 2,000 non-targeting control sgRNAs and 4,500 control-gene-targeting sgRNAs for a total of 71,878 sgRNAs. It can be used for genome-scale screening in the mouse liver.

• Vector Backbone:

  pLentiCRISPRv2-Stuffer-HepmCherry (Plasmid #192825) - does not express Cas9

• Depositing Labs: Kristin Knouse
• Publication: Keys and Knouse. Cell Genom. 2022 Dec 14;2(12):100217. doi: 10.1016/j.xgen.2022.100217.

Library Details

• Species: Mouse
• Genes targeted: 13,189
• gRNAs: 71,878
• Controls:
  
  • 6,500 total
  • 2,000 non-targeting
  • 4,500 targeting control genes
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (PDF, 101 KB)
  • Library Sequencing Protocol (PDF, 89 KB)
• Depositor Data:
  • Illumina Sequencing Results (XLSX, 1.8 KB)
  • sgRNA Sequences (XLSX, 2.9 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA
• Takara Bio Limited Use Label License (formerly Clontech)

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.01.30.428976 for bioRxiv preprint.