Young Lab - openCIDAR MoClo Toolkit
(Kit #
1000000227
)
Depositing Lab: Eric Young
This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota, such as Escherichia coli, Pseudomonas putida, Cupriavidus necator, and Komagataeibacter nataicola.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria. Keating K, Young EM. ACS Synth Biol. 2023 Jul 21;12(7):2061-2072. doi: 10.1021/acssynbio.3c00104. PubMed (Link opens in a new window) Article (Link opens in a new window).
Description
This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota. The vectors contain resistance cassettes for kanamycin, chloramphenicol, and spectinomycin. A combinatorial library of constructs expressing GFP at 18 levels for rapid characterization of expression parts in new organisms is also included.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The openCIDAR MoClo Toolkit was a gift from Eric Young (Addgene kit #1000000227).”
For your Reference section:
Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria. Keating K, Young EM. ACS Synth Biol. 2023 Jul 21;12(7):2061-2072. doi: 10.1021/acssynbio.3c00104. PubMed (Link opens in a new window) Article (Link opens in a new window).
openCIDAR MoClo Toolkit - #1000000227
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Kanamycin | |
| Spectinomycin | |
| Chloramphenicol |