CIDAR MoClo Parts Kit
(Kit #
1000000059
)
Depositing Labs:
Douglas Densmore
The Cross-disciplinary Integration of Design Automation Research lab (CIDAR) MoClo Library is collection of modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design and expression tuning in E. coli.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Iverson SV, Haddock TL, Beal J, Densmore DM. ACS Synth Biol. 2015 Nov 4. doi: 10.1002/bit.25814. PubMed PMID 26479688.
Description
The CIDAR MoClo Library contains promoters, ribosomal binding sites, coding sequence, terminators, vectors, and a set of fluorescent control plasmids. Optimized protocols reduce reaction time and cost by >80% from previously published protocols.
Kit Documentation
Download a ZIP file containing the plasmid sequences in GenBank format:
Documents describing this kit:
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059).”
For your Reference section:
CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Iverson SV, Haddock TL, Beal J, Densmore DM. ACS Synth Biol. 2015 Nov 4. doi: 10.1002/bit.25814. PubMed PMID 26479688.
CIDAR MoClo Parts Kit - #1000000059
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Kanamycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | J23100_AB | Ampicillin |
| A / 2 | J23100_EB | Ampicillin |
| A / 3 | J23100_FB | Ampicillin |
| A / 4 | J23100_GB | Ampicillin |
| A / 5 | J23102_AB | Ampicillin |
| A / 6 | J23102_EB | Ampicillin |
| A / 7 | J23102_FB | Ampicillin |
| A / 8 | J23102_GB | Ampicillin |
| A / 9 | J23103_AB | Ampicillin |
| A / 10 | J23103_EB | Ampicillin |
| A / 11 | J23103_FB | Ampicillin |
| A / 12 | J23103_GB | Ampicillin |
| B / 1 | J23106_AB | Ampicillin |
| B / 2 | J23106_EB | Ampicillin |
| B / 3 | J23106_FB | Ampicillin |
| B / 4 | J23106_GB | Ampicillin |
| B / 5 | J23107_AB | Ampicillin |
| B / 6 | J23107_EB | Ampicillin |
| B / 7 | J23107_FB | Ampicillin |
| B / 8 | J23107_GB | Ampicillin |
| B / 9 | J23116_AB | Ampicillin |
| B / 10 | J23116_EB | Ampicillin |
| B / 11 | J23116_FB | Ampicillin |
| B / 12 | J23116_GB | Ampicillin |
| C / 1 | R0010 (pLacI)_AB | Ampicillin |
| C / 2 | R0010 (pLacI)_EB | Ampicillin |
| C / 3 | R0010 (pLacI)_FB | Ampicillin |
| C / 4 | R0010 (pLacI)_GB | Ampicillin |
| C / 5 | R0040 (pTet)_AB | Ampicillin |
| C / 6 | R0040 (pTet)_EB | Ampicillin |
| C / 7 | R0040 (pTet)_FB | Ampicillin |
| C / 8 | R0040 (pTet)_GB | Ampicillin |
| C / 9 | R0063 (pLuxR-pR)_AB | Ampicillin |
| C / 10 | R0063 (pLuxR-pR)_EB | Ampicillin |
| C / 11 | R0063 (pLuxR-pR)_FB | Ampicillin |
| C / 12 | R0063 (pLuxR-pR)_GB | Ampicillin |
| D / 1 | I13453 (pBAD)_AB | Ampicillin |
| D / 2 | I13453 (pBAD)_EB | Ampicillin |
| D / 3 | I13453 (pBAD)_FB | Ampicillin |
| D / 4 | I13453 (pBAD)_GB | Ampicillin |
| D / 5 | B0032m_BC | Ampicillin |
| D / 6 | B0033m_BC | Ampicillin |
| D / 7 | B0034m_BC | Ampicillin |
| D / 8 | BCD12_BC | Ampicillin |
| D / 9 | BCD2_BC | Ampicillin |
| D / 10 | BCD8_BC | Ampicillin |
| D / 11 | C0012m (lacI)_CD | Ampicillin |
| D / 12 | C0040 (tetR)_CD | Ampicillin |
| E / 1 | C0062 (luxR)_CD | Ampicillin |
| E / 2 | C0080 (araC)_CD | Ampicillin |
| E / 3 | cre_CD | Ampicillin |
| E / 4 | E0030 (YFP)_CD | Ampicillin |
| E / 5 | E0040m (GFP)_CD | Ampicillin |
| E / 6 | E1010m (RFP)_CD | Ampicillin |
| E / 7 | eBFP2 (eBFP2)_CD | Ampicillin |
| E / 8 | B0015_DE | Ampicillin |
| E / 9 | B0015_DF | Ampicillin |
| E / 10 | B0015_DG | Ampicillin |
| E / 11 | B0015_DH | Ampicillin |
| E / 12 | DVA_AB | Ampicillin |
| F / 1 | DVA_AE | Ampicillin |
| F / 2 | DVA_AF | Ampicillin |
| F / 3 | DVA_AG | Ampicillin |
| F / 4 | DVA_AH | Ampicillin |
| F / 5 | DVA_BC | Ampicillin |
| F / 6 | DVA_CD | Ampicillin |
| F / 7 | DVA_DE | Ampicillin |
| F / 8 | DVA_DF | Ampicillin |
| F / 9 | DVA_DG | Ampicillin |
| F / 10 | DVA_DH | Ampicillin |
| F / 11 | DVA_EB | Ampicillin |
| F / 12 | DVA_EF | Ampicillin |
| G / 1 | DVA_EG | Ampicillin |
| G / 2 | DVA_EH | Ampicillin |
| G / 3 | DVA_FB | Ampicillin |
| G / 4 | DVA_GB | Ampicillin |
| G / 5 | DVA | Ampicillin |
| G / 6 | pJ02B2Gm_AE | Ampicillin |
| G / 7 | pJ02B2Gm_EF | Ampicillin |
| G / 8 | pJ02B2Rm_AE | Ampicillin |
| G / 9 | pJ02B2Rm_EF | Ampicillin |
| G / 10 | pJ02B2Gm:Rm_AF | Ampicillin |
| G / 11 | pJ02B2Rm:Gm_AF | Ampicillin |
| G / 12 | pJ02B2Gm_AE | Kanamycin |
| H / 1 | pJ02B2Gm_EF | Kanamycin |
| H / 2 | pJ02B2Rm_AE | Kanamycin |
| H / 3 | pJ02B2Rm_EF | Kanamycin |
| H / 5 | DVK_AF | Kanamycin |
| H / 7 | DVK_FG | Kanamycin |
| H / 8 | DVK_GH | Kanamycin |
| H / 9 | DVK | Kanamycin |