Marx Lab FREQ-Seq Kit: High Resolution Allele Frequency Measurements
(Kit #
1000000032
)
Depositing Labs: Christopher Marx
The FREQ-Seq collection can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. By counting DNA sequence reads, this strategy allows for direct quantitative allele detection.
This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°Celsius immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Original Publication
FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012; 7(10): e47959. PubMed . Open Access Article .Description
The FREQ-Seq collection can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. By counting DNA sequence reads, this strategy allows for direct quantitative allele detection. The kit consists of a 48 plasmid adaptor library, with each plasmid carrying a unique barcoded Illumina-M13F bridging primer. These bridging primers can be amplified and used to generate Illumina sequencing libraries by means of a 2-step PCR-based protocol and are compatible with single-end or paired-end read flow cells.
Kit Documentation
FREQ-Seq kit.pdf 1.3Â MBProtocols
An abbreviated FREQ-Seq Protocol from the publication:
marx-lab-freq-seq-protocol.pdf 316.9Â KBLinks
Analysis software: FREQ-Seq Website
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The FREQ-seq plasmids were a gift from Christopher Marx (Addgene kit # 1000000032)"
For your Reference section:
FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012; 7(10): e47959.
PubMed
.
Open Access Article
.
FREQ-Seq Kit - #1000000032
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pFSBC1 | Ampicillin |
| A / 2 | pFSBC2 | Ampicillin |
| A / 3 | pFSBC3 | Ampicillin |
| A / 4 | pFSBC4 | Ampicillin |
| A / 5 | pFSBC5 | Ampicillin |
| A / 6 | pFSBC6 | Ampicillin |
| A / 7 | pFSBC7 | Ampicillin |
| A / 8 | pFSBC8 | Ampicillin |
| A / 9 | pFSBC9 | Ampicillin |
| A / 10 | pFSBC10 | Ampicillin |
| A / 11 | pFSBC11 | Ampicillin |
| A / 12 | pFSBC12 | Ampicillin |
| B / 1 | pFSBC13 | Ampicillin |
| B / 2 | pFSBC14 | Ampicillin |
| B / 3 | pFSBC15 | Ampicillin |
| B / 4 | pFSBC16 | Ampicillin |
| B / 5 | pFSBC17 | Ampicillin |
| B / 6 | pFSBC18 | Ampicillin |
| B / 7 | pFSBC19 | Ampicillin |
| B / 8 | pFSBC20 | Ampicillin |
| B / 9 | pFSBC21 | Ampicillin |
| B / 10 | pFSBC22 | Ampicillin |
| B / 11 | pFSBC23 | Ampicillin |
| B / 12 | pFSBC24 | Ampicillin |
| C / 1 | pFSBC25 | Ampicillin |
| C / 2 | pFSBC26 | Ampicillin |
| C / 3 | pFSBC27 | Ampicillin |
| C / 4 | pFSBC28 | Ampicillin |
| C / 5 | pFSBC29 | Ampicillin |
| C / 6 | pFSBC30 | Ampicillin |
| C / 7 | pFSBC31 | Ampicillin |
| C / 8 | pFSBC32 | Ampicillin |
| C / 9 | pFSBC33 | Ampicillin |
| C / 10 | pFSBC34 | Ampicillin |
| C / 11 | pFSBC35 | Ampicillin |
| C / 12 | pFSBC36 | Ampicillin |
| D / 1 | pFSBC37 | Ampicillin |
| D / 2 | pFSBC38 | Ampicillin |
| D / 3 | pFSBC39 | Ampicillin |
| D / 4 | pFSBC40 | Ampicillin |
| D / 5 | pFSBC41 | Ampicillin |
| D / 6 | pFSBC42 | Ampicillin |
| D / 7 | pFSBC43 | Ampicillin |
| D / 8 | pFSBC44 | Ampicillin |
| D / 9 | pFSBC45 | Ampicillin |
| D / 10 | pFSBC46 | Ampicillin |
| D / 11 | pFSBC47 | Ampicillin |
| D / 12 | pFSBC48 | Ampicillin |