SapTrap CRISPR/Cas Toolkit
(Kit #
1000000077
)
Depositing Lab: Erik Jorgensen
The SapTrap Toolkit is a collection of plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. [Epub ahead of print] PubMed PMID: 26837755 .
Description
SapTrap is a modular toolkit for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector.
Depositor-provided Files
The Jorgensen Lab has provided a methods file that includes information about:
- Oligo design tips and explanatory figures for SapTrap vectors
- SapTrap assembly procedure
- Protocol for injection and isolation
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The SapTrap kit was a gift from Erik Jorgensen (Addgene kit # 1000000077).”
For your Reference section:
SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. [Epub ahead of print] PubMed PMID: 26837755 .
SapTrap Kit - #1000000077
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Kanamycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pMLS134 | Ampicillin |
| A / 2 | pMLS256 | Ampicillin |
| A / 3 | pMLS257 | Ampicillin |
| A / 4 | pMLS328 | Ampicillin |
| A / 5 | pMLS262 | Ampicillin |
| A / 6 | pMLS338 | Ampicillin |
| A / 7 | pMLS252 | Kanamycin |
| A / 8 | pMLS254 | Kanamycin |
| A / 9 | pMLS271 | Kanamycin |
| A / 10 | pMLS286 | Kanamycin |
| A / 11 | pMLS291 | Kanamycin |
| A / 12 | pMLS292 | Kanamycin |
| B / 1 | pMLS268 | Kanamycin |
| B / 2 | pMLS269 | Kanamycin |
| B / 3 | pMLS272 | Kanamycin |
| B / 4 | pMLS279 | Kanamycin |
| B / 5 | pMLS287 | Kanamycin |
| B / 6 | pMLS382 | Kanamycin |
| B / 7 | pMLS281 | Kanamycin |
| B / 8 | pMLS282 | Kanamycin |
| B / 9 | pMLS285 | Kanamycin |
| B / 10 | pMLS288 | Kanamycin |
| B / 11 | pMLS383 | Kanamycin |
| B / 12 | pMLS297 | Kanamycin |
| C / 1 | pMLS280 | Kanamycin |
| C / 2 | pWD509 | Kanamycin |