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An expanded auxin-inducible degron toolkit for Caenorhabditis elegans.

Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 10.1093/genetics/iyab006. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
121054pJW1583GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
121055pJW1584YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
121057pJW1586mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
121059pJW1592GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
121061pJW1594TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
121062pJW1595mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
154310pJW1347Donor-cassette for SapTrap
154311pJW1348NT Donor-cassette for SapTrap
154312pJW1582mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
154314pJW1659NT Donor-cassette for SapTrap
154315pJW1759NT Donor-cassette for SapTrap
154316pJW1787NT Donor-cassette for SapTrap
154317pJW1788CT Donor-cassette for SapTrap
154318pJW1805CT Donor-cassette for SapTrap
154319pJW1806NT Donor-cassette for SapTrap
154320pJW1816Multi-cassette for SapTrap
154321pJW1820Multi-cassette for SapTrap
154322pJW1822Multi-cassette for SapTrap
154323pJW1827Multi-cassette for SapTrap
154324pJW1838sgRNA Destination Vector for SapTrap
154325pJW1839sgRNA Destination Vector for SapTrap
154326pJW1846Multi-cassette for SapTrap
154327pJW1849sgRNA Target Vector for CRISPR
154328pJW1850sgRNA Target Vector for CRISPR
154329pJW1851sgRNA Target Vector for CRISPR
154330pJW1882sgRNA Target Vector for CRISPR
154331pJW1883sgRNA Target Vector for CRISPR
154332pJW1884sgRNA Target Vector for CRISPR
154333pJW1886Multi-cassette for SapTrap
154334pJW2072Crispr repair template: add insertion site specific homology arms by PCR before insertion
154335pJW2086Crispr repair template: add insertion site specific homology arms by PCR before insertion
154336pJW2088Crispr repair template: add insertion site specific homology arms by PCR before insertion
154337pJW1836Promoter reporter
154338pJW1841Promoter reporter
154339pJW1947Enhancer reporter
154340pJW1948Enhancer reporter
163092pJW1821CRISPR repair template: add insertion site specific homology arms by PCR before insertion
163093pJW1927CRISPR repair template: add insertion site specific homology arms by PCR before insertion
163094pJW2098CRISPR repair template: add insertion site specific homology arms by PCR before insertion
163095pJW2171CRISPR repair template: add insertion site specific homology arms by PCR before insertion
163096pJW2172CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Antibodies from Article