Zebrafish Monoclonal Antibodies

This is a collection of plasmids that encode monoclonal antibodies to zebrafish cell surface receptors and secreted proteins. The plasmids encode both the light and heavy chains of mouse monoclonal antibodies to zebrafish proteins and the antibodies are produced by transfecting mammalian cell lines and collecting the secreted monoclonal antibody from the supernatant.

Please see the following reference for more information:

A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C et al., BMC Biol. 2010 Jun 4;8:76. PubMed 20525357

Plasmid ID	Plasmid Name	Zebrafish Gene
28214	SI1-Unc5b	Unc5b
28215	SI2-Unc5b	Unc5b
28216	SI4-Jamc.2	Jamc.2
28217	SI3-Flrt3	Flrt3
36115	SI5-IGSF21a	IGSF21a
36141	SI6-IGSF21a	IGSF21a
36142	SI7-NCAM2	NCAM2
36143	SI8-IGSF21b	IGSF21b
36144	SI9-ROBO1	ROBO1
46300	SI10-Brevican	Brevican
46301	SI11-Sema4c	Sema4c
46302	SI12-Lrrc3b	Lrrc3b
46303	SI13-Lrrc3b	Lrrc3b
46304	SI14-Kit b	Kit b
46305	SI15-CD276	CD276
58948	SI16-Kita	Kit a
58949	pVk7-33G1-BioLHis	IGHC1
58950	pVk7-33G1-Flag	IGHC1