CRISPR Plasmids: Interfere
Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus.
Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.
Browse, sort, or search the tables below for CRISPR plasmids for transcriptional inhibition.Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast.
Mammalian
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Bacteria
| Plasmid | Gene/Insert | Promoter | PI | Publication |
|---|
Plant
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Yeast
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!