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CRISPR Plasmids: Activate

Browse CRISPR Plasmids

By Function
Genome Editing
Transcriptional Regulation
RNA Targeting
Other Applications
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gRNAs
By Species

CRISPR Resources

Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.




Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.

Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.

Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Bacteria

Plasmid Gene/Insert Promoter PI Publication

Drosophila

Plasmid Gene/Insert Promoter PI Publication

Plant

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

C. elegans

Plasmid Gene/Insert Promoter PI Publication

Yeast

Plasmid Gene/Insert Promoter Selectable Marker PI Publication


Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!