Dankort Lab pLEG/pREG Modular Viral Vector System
The LEG (Lentiviral Expression Gateway) is system a flexible vector system to allow the rapid production of lentiviral and retroviral (REG) vectors that express cDNAs with or without inhibitory RNAs (shRNAmirs) using Gateway technology. Click here to see full description.
These plasmids are originally described in:
A Modular Lentiviral and Retroviral Construction System to Rapidly Generate Vectors for Gene Expression and Gene Knockdown In Vitro and In Vivo. Geiling, B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett, S, Dankort D. PLoS ONE 8(10): e76279. doi:10.1371/journal.pone.0076279.
Plasmids can be ordered via the links below:
| ID | Plasmid | Purpose |
|---|---|---|
| 48942 | pBEG R1-ChlorR-R3 #BGV148: | Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectors |
| 48943 | pBEG R1-ChlorR-R4 #BGV049: | Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors. |
| 48952 | pBEG R2-L3 #BGV193: | For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid). |
| 48953 | pBEG R3-miRNA(ccdB)-L4 #BGV228: | To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid). |
| 48954 | pBEG R3-L4 #BGV194: | For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid). |
| 48955 | pCheck2 Dest (R1-R2) #BGV129: | Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors. |
| 48956 | pLEG(R1-R3) #BGV149: | Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids). |
| 48957 | pLEG(R1-R4) #BGV057: | Lentiviral Destination vector containing AttR1-AttR4 site. For use with four-way LR reaction with cDNA in entry vector, a marker in pBEG R2-L3 plasmids and an shRNA in pBEG R3-L4 plasmid (module 2, module 3 and module 4 plasmids). |
| 48958 | pREG(R1-R3) #BGV141: | Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids). |
| 48973 | pBEG R2-dsRed-L3 #BGV203: | Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48977 | pBEG R2-eCFP-L3 #BGV202: | Supplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48979 | pBEG R2-eGFP-L3 #BGV201: | Supplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48982 | pBEG R2-iBlast-L3 #BGV204: | Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48985 | pBEG R2-iHygro-L3 #BGV206: | Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48986 | pBEG R2-iNeo-L3 #BGV207: | Supplies the selection marker (neomycin/G418 resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48987 | pBEG R2-iPuro-L3 #BGV205: | Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |
| 48991 | pREG(R1-R4) #BGV059: | Retroviral Destination vector containing AttR1-AttR4 site. For use with four-way LR reaction with cDNA in entry vector, a marker in pBEG R2-L3 plasmids and an shRNA in pBEG R3-L4 plasmid (module 2, module 3 and module 4 plasmids). |
| 48992 | pBEG R2-iCreT2ALuc-L3 #BGV208: | Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid). |