Addgene BasiginCD4d3+4-Comp-His-Stop - Sequence Analyzer Skip to main content
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Sequence Analyzer: BasiginCD4d3+4-Comp-His-Stop


Map View

AscI - BssHII (7696) PluTI (6949) SfoI (6947) NarI (6946) KasI (6945) BmtI (6787) NheI (6783) Kozak sequence EagI - NotI (6718) PmeI (6711) pMT2-F (6670 .. 6689) BsmBI - Esp3I (6484) BlpI (6401) PaeR7I - XhoI (6361) LNCX (6165 .. 6189) CMV-F (6119 .. 6139) CMV promoter BsaAI - SnaBI (5940) BsrGI (5543) M13 rev M13 Reverse (5298 .. 5314) lac operator M13/pUC Reverse (5279 .. 5301) CAP binding site BspQI - SapI (5085) L4440 (5066 .. 5083) pBR322ori-F (4813 .. 4832) NmeAIII (3933) FspI (3857) SbfI (84) CsiI - SexAI * (312) KflI (371) EcoRI (550) BspEI (566) Acc65I (575) AgeI (578) KpnI (579) BmgBI (646) XbaI (724) 6xHis BamHI (785) rbglobpA-R (865 .. 884) BspDI * - ClaI * (933) DraIII (1140) BstAPI (1164) PasI (1253) HpaI (1372) Bsu36I (1498) EcoRV (1512) BsaBI (1914) MluI (2188) pBRforEco (3102 .. 3120) XmnI (3480) Amp-R (3511 .. 3530) PvuI (3711) BasiginCD4d3+4-Comp-His-Stop 7702 bp

Sequence View

60 VDLHHGL* STSITAYKSEGESAEFSFPL DVEM g t c g a c c t c c a t c a c g g c c t a t a a g a g t g a g g g g g a g t c a g c g g a g t t c t c c t t c c c a c t c a g c t g g a g g t a g t g c c g g a t a t t c t c a c t c c c c c t c a g t c g c c t c a a g a g g a a g g g t g a

SbfI 120 NLGEESLQGELRWKAEKAPS c a a c c t t g g a g a g g a a a g c c t g c a g g g a g a g t t g a g a t g g a a g g c a g a g a a g g c t c c t t c g t t g g a a c c t c t c c t t t c g g a c g t c c c t c t c a a c t c t a c c t t c c g t c t c t t c c g a g g a a g

180 SQSWITFSLKNQKVSVQKST t t c c c a g t c c t g g a t c a c c t t c t c c c t a a a g a a c c a a a a g g t g t c t g t g c a g a a g t c t a c a a g g g t c a g g a c c t a g t g g a a g a g g g a t t t c t t g g t t t t c c a c a g a c a c g t c t t c a g a t g

240 SNPKFQLSETLPLTLQIPQV t a g c a a c c c c a a g t t c c a g c t g t c c g a a a c g c t c c c a c t c a c c c t t c a g a t a c c c c a g g t a t c g t t g g g g t t c a a g g t c g a c a g g c t t t g c g a g g g t g a g t g g g a a g t c t a t g g g g t c c a

300 SLQFAGSGNLTLTLDRGILY c t c c c t t c a g t t t g c t g g t t c t g g c a a c c t g a c c c t g a c t c t g g a c a g a g g g a t a c t g t a g a g g g a a g t c a a a c g a c c a a g a c c g t t g g a c t g g g a c t g a g a c c t g t c t c c c t a t g a c a t

SexAI*CsiI 360 QEVNLVVMKVTQPDSNTLTC t c a g g a a g t g a a c c t g g t g g t g a t g a a a g t g a c t c a g c c c g a c a g c a a c a c t t t g a c c t g a g t c c t t c a c t t g g a c c a c c a c t a c t t t c a c t g a g t c g g g c t g t c g t t g t g a a a c t g g a c

KflI 420 EVMGPTSPKMRLILKQENQE t g a g g t g a t g g g a c c c a c c t c a c c c a a g a t g a g a c t g a t c t t g a a g c a g g a g a a t c a g g a a c t c c a c t a c c c t g g g t g g a g t g g g t t c t a c t c t g a c t a g a a c t t c g t c c t c t t a g t c c t

480 ARVSRQEKVIQVQAPEAGVW g g c c a g g g t c t c c a g g c a g g a g a a a g t g a t t c a a g t g c a g g c c c c t g a a g c a g g g g t g t g c c g g t c c c a g a g g t c c g t c c t c t t t c a c t a a g t t c a c g t c c g g g g a c t t c g t c c c c a c a c

540 QCLLSEGEEVKMDSKIQVLS g c a a t g t c t a c t g a g t g a a g g t g a a g a g g t c a a g a t g g a c t c c a a g a t c c a g g t t t t a t c c g t t a c a g a t g a c t c a c t t c c a c t t c t c c a g t t c t a c c t g a g g t t c t a g g t c c a a a a t a g

Acc65IAgeIKpnIBspEIEcoRI 600 KGLNSGGGSGGGTGGGDLAP c a a a g g g t t g a a t t c c g g c g g c g g c t c c g g a g g c g g t a c c g g t g g c g g c g a c c t a g c c c c g t t t c c c a a c t t a a g g c c g c c g c c g a g g c c t c c g c c a t g g c c a c c g c c g c t g g a t c g g g g

BmgBI 660 QMLRELQETNAALQDVRELL a c a g a t g c t t c g a g a a c t c c a g g a g a c t a a t g c g g c g c t g c a a g a c g t g a g a g a g c t c t t t g t c t a c g a a g c t c t t g a g g t c c t c t g a t t a c g c c g c g a c g t t c t g c a c t c t c t c g a g a a

720 RQQVKEITFLKNTVMECDAC g c g a c a g c a g g t c a a g g a g a t c a c c t t c c t g a a g a a t a c g g t g a t g g a a t g t g a c g c c t g c g c t g t c g t c c a g t t c c t c t a g t g g a a g g a c t t c t t a t g c c a c t a c c t t a c a c t g c g g a c

XbaI 780 1 5 HHHHHH 6xHis GLDRNLPPLAPLGPHHHHHH c g g t c t a g a c a g g a a c c t g c c t c c t c t g g c c c c t c t g g g c c c c c a c c a c c a t c a c c a c c a g c c a g a t c t g t c c t t g g a c g g a g g a g a c c g g g g a g a c c c g g g g g t g g t g g t a g t g g t g g t

BamHI 840 * 6xHis β- globin poly(A) signal * t t g a g g a t c c c c c g a c c t c g a c c t c t g g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g a a c t c c t a g g g g g c t g g a g c t g g a g a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c

900 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C C C rbglobpA-R t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t g g a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a c c

BspDI*ClaI* 960 t c g a g a t c c c t c g g a g a t c t c t a g c t a g a g g a t c g a t c c c c g c c c c g g a c g a a c t a a a c c a g c t c t a g g g a g c c t c t a g a g a t c g a t c t c c t a g c t a g g g g c g g g g c c t g c t t g a t t t g g

1020 t g a c t a c g a c a t c t c t g c c c c t t c t t c g c g g g g c a g t g c a t g t a a t c c c t t c a g t t g g t t a c t g a t g c t g t a g a g a c g g g g a a g a a g c g c c c c g t c a c g t a c a t t a g g g a a g t c a a c c a a

1080 g g t a c a a c t t g c c a a c t g g g c c c t g t t c c a c a t g t g a c a c g g g g g g g g a c c a a a c a c a a a c c a t g t t g a a c g g t t g a c c c g g g a c a a g g t g t a c a c t g t g c c c c c c c c t g g t t t g t g t t t

DraIII 1140 g g g g t t c t c t g a c t g t a g t t g a c a t c c t t a t a a a t g g a t g t g c a c a t t t g c c a a c a c t g a c c c c a a g a g a c t g a c a t c a a c t g t a g g a a t a t t t a c c t a c a c g t g t a a a c g g t t g t g a c t

BstAPI 1200 g t g g c t t t c a t c c t g g a g c a g a c t t t g c a g t c t g t g g a c t g c a a c a c a a c a t t g c c t t t a c a c c g a a a g t a g g a c c t c g t c t g a a a c g t c a g a c a c c t g a c g t t g t g t t g t a a c g g a a a t

PasI 1260 t g t g t a a c t c t t g g c t g a a g c t c t t a c a c c a a t g c t g g g g g a c a t g t a c c t c c c a g g g g c a c a c a t t g a g a a c c g a c t t c g a g a a t g t g g t t a c g a c c c c c t g t a c a t g g a g g g t c c c c g

1320 c c a g g a a g a c t a c g g g a g g c t a c a c c a a c g t c a a t c a g a g g g g c c t g t g t a g c t a c c g a t g g t c c t t c t g a t g c c c t c c g a t g t g g t t g c a g t t a g t c t c c c c g g a c a c a t c g a t g g c t a

HpaI 1380 a a g c g g a c c c t c a a g a g g g c a t t a g c a a t a g t g t t t a t a a g g c c c c c t t g t t a a c c c t a a t t c g c c t g g g a g t t c t c c c g t a a t c g t t a t c a c a a a t a t t c c g g g g g a a c a a t t g g g a t t

1440 a c g g g t a g c a t a t g c t t c c c g g g t a g t a g t a t a t a c t a t c c a g a c t a a c c c t a a t t c a a t t g c c c a t c g t a t a c g a a g g g c c c a t c a t c a t a t a t g a t a g g t c t g a t t g g g a t t a a g t t a

Bsu36I 1500 a g c a t a t g t t a c c c a a c g g g a a g c a t a t g c t a t c g a a t t a g g g t t a g t a a a a g g g t c c t a t c g t a t a c a a t g g g t t g c c c t t c g t a t a c g a t a g c t t a a t c c c a a t c a t t t t c c c a g g a t

EcoRV 1560 a g g a a c a g c g a t a t c t c c c a c c c c a t g a g c t g t c a c g g t t t t a t t t a c a t g g g g t c a g g a t c c t t g t c g c t a t a g a g g g t g g g g t a c t c g a c a g t g c c a a a a t a a a t g t a c c c c a g t c c t

1620 t t c c a c g a g g g t a g t g a a c c a t t t t a g t c a c a a g g g c a g t g g c t g a a g a t c a a g g a g c g g a a g g t g c t c c c a t c a c t t g g t a a a a t c a g t g t t c c c g t c a c c g a c t t c t a g t t c c t c g c c

1680 g c a g t g a a c t c t c c t g a a t c t t c g c c t g c t t c t t c a t t c t c c t t c g t t t a g c t a a t a g a a c g t c a c t t g a g a g g a c t t a g a a g c g g a c g a a g a a g t a a g a g g a a g c a a a t c g a t t a t c t t

1740 t a a c t g c t g a g t t g t g a a c a g t a a g g t g t a t g t g a g g t g c t c g a a a a c a a g g t t t c a g g t a t t g a c g a c t c a a c a c t t g t c a t t c c a c a t a c a c t c c a c g a g c t t t t g t t c c a a a g t c c a

1800 g a c g c c c c c a g a a t a a a a t t t g g a c g g g g g g t t c a g t g g t g g c a t t g t g c t a t g a c a c c a c t g c g g g g g t c t t a t t t t a a a c c t g c c c c c c a a g t c a c c a c c g t a a c a c g a t a c t g t g g t

1860 a t a t a a c c c t c a c a a a c c c c t t g g g c a a t a a a t a c t a g t g t a g g a a t g a a a c a t t c t g a a t a t a t t g g g a g t g t t t g g g g a a c c c g t t a t t t a t g a t c a c a t c c t t a c t t t g t a a g a c t t

BsaBI 1920 t a t c t t t a a c a a t a g a a a t c c a t g g g g t g g g g a c a a g c c g t a a a g a c t g g a t g t c c a t c t a t a g a a a t t g t t a t c t t t a g g t a c c c c a c c c c t g t t c g g c a t t t c t g a c c t a c a g g t a g a

1980 c a c a c g a a t t t a t g g c t a t g g g c a a c a c a t a a t c c t a g t g c a a t a t g a t a c t g g g g t t a t g t g t g c t t a a a t a c c g a t a c c c g t t g t g t a t t a g g a t c a c g t t a t a c t a t g a c c c c a a t a

2040 t a a g a t g t g t c c c a g g c a g g g a c c a a g a c a g g t g a a c c a t g t t g t t a c a c t c t a t t t g t a a t t c t a c a c a g g g t c c g t c c c t g g t t c t g t c c a c t t g g t a c a a c a a t g t g a g a t a a a c a t

2100 a c a a g g g g a a a g a g a g t g g a c g c c g a c a g c a g c g g a c t c c a c t g g t t g t c t c t a a c a c c c t g t t c c c c t t t c t c t c a c c t g c g g c t g t c g t c g c c t g a g g t g a c c a a c a g a g a t t g t g g g

2160 c c g a a a a t t a a a c g g g g c t c c a c g c c a a t g g g g c c c a t a a a c a a a g a c a a g t g g c c a c t c g g c t t t t a a t t t g c c c c g a g g t g c g g t t a c c c c g g g t a t t t g t t t c t g t t c a c c g g t g a g

MluI 2220 t t t t t t t t g a a a t t g t g g a g t g g g g g c a c g c g t c a g c c c c c a c a c g c c g c c c t g c g g t t t a a a a a a a a c t t t a a c a c c t c a c c c c c g t g c g c a g t c g g g g g t g t g c g g c g g g a c g c c a a a

2280 t g g a c t g t a a a a t a a g g g t g t a a t a a c t t g g c t g a t t g t a a c c c c g c t a a c c a c t g c g g t a c c t g a c a t t t t a t t c c c a c a t t a t t g a a c c g a c t a a c a t t g g g g c g a t t g g t g a c g c c a

2340 c a a a c c a c t t g c c c a c a a a a c c a c t a a t g g c a c c c c g g g g a a t a c c t g c a t a a g t a g g t g g t t t g g t g a a c g g g t g t t t t g g t g a t t a c c g t g g g g c c c c t t a t g g a c g t a t t c a t c c a c

2400 g g c g g g c c a a g a t a g g g g c g c g a t t g c t g c g a t c t g g a g g a c a a a t t a c a c a c a c t t g c g c c g c c c g g t t c t a t c c c c g c g c t a a c g a c g c t a g a c c t c c t g t t t a a t g t g t g t g a a c g c

2460 c c t g a g c g c c a a g c a c a g g g t t g t t g g t c c t c a t a t t c a c g a g g t c g c t g a g a g c a c g g t g g a c t c g c g g t t c g t g t c c c a a c a a c c a g g a g t a t a a g t g c t c c a g c g a c t c t c g t g c c a

2520 g g g c t a a t g t t g c c a t g g g t a g c a t a t a c t a c c c a a a t a t c t g g a t a g c a t a t g c t a t c c c c c g a t t a c a a c g g t a c c c a t c g t a t a t g a t g g g t t t a t a g a c c t a t c g t a t a c g a t a g g

2580 t a a t c t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c a t t a g a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g

2640 t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a g g c t a t c c a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t c c g a t a g g

2700 t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g

2760 t a a t c t g t a t c c g g g t a g c a t a t g c t a t c c t a a t a g a g a t t a g g g t a g t a t a t g c t a t c c a t t a g a c a t a g g c c c a t c g t a t a c g a t a g g a t t a t c t c t a a t c c c a t c a t a t a c g a t a g g

2820 t a a t t t a t a t c t g g g t a g c a t a t a c t a c c c a a a t a t c t g g a t a g c a t a t g c t a t c c t a a t a t t a a a t a t a g a c c c a t c g t a t a t g a t g g g t t t a t a g a c c t a t c g t a t a c g a t a g g a t t a

2880 c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a g g c t a t c c t a a t g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t c c g a t a g g a t t a

2940 c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a

3000 t t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t a a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a

3060 c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t c c g g g t a g c a t a t g c t a t c c t c a t g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a g g c c c a t c g t a t a c g a t a g g a g t a

3120 C G G A G C A C T A T G C G G A T A A pBRforEco g a t a a g c t g t c a a a c a t g a g a a t t t t c t t g a a g a c g a a a g g g c c t c g t g a t a c g c c t a t t c t a t t c g a c a g t t t g t a c t c t t a a a a g a a c t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a

3180 t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c

3240 AmpR promoter a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a

3300 1 signal sequence MSI AmpR promoter AmpR MSI c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a

3360 5 10 15 20 signal sequence QHFRVALIPFFAAFCLPVFA AmpR QHFRVALIPFFAAFCLPVFA t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g

3420 25 30 35 40 HPETLVKVKDAEDQLGARVG AmpR HPETLVKVKDAEDQLGARVG t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c

XmnI 3480 45 50 55 60 YIELDLNSGKILESFRPEER AmpR YIELDLNSGKILESFRPEER t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c

3540 65 70 75 80 FPMMSTFKVLLCGAVLSRVD AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FPMMSTFKVLLCGAVLSRVD t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t

3600 85 90 95 100 AGQEQLGRRIHYSQNDLVEY AmpR AGQEQLGRRIHYSQNDLVEY c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t

3660 105 110 115 120 SPVTEKHLTDGMTVRELCSA AmpR SPVTEKHLTDGMTVRELCSA c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g

PvuI 3720 125 130 135 140 AITMSDNTAANLLLTTIGGP AmpR AITMSDNTAANLLLTTIGGP t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g

3780 145 150 155 160 KELTAFLHNMGDHVTRLDRW AmpR KELTAFLHNMGDHVTRLDRW g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c

3840 165 170 175 180 EPELNEAIPNDERDTTMPAA AmpR EPELNEAIPNDERDTTMPAA g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g c a g c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c g t c g

FspI 3900 185 190 195 200 MATTLRKLLTGELLTLASRQ AmpR MATTLRKLLTGELLTLASRQ a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t

NmeAIII 3960 205 210 215 220 QLIDWMEADKVAGPLLRSAL AmpR QLIDWMEADKVAGPLLRSAL a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a

4020 225 230 235 240 PAGWFIADKSGAGERGSRGI AmpR PAGWFIADKSGAGERGSRGI t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a

4080 245 250 255 260 IAALGPDGKPSRIVVIYTTG AmpR IAALGPDGKPSRIVVIYTTG c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c

4140 265 270 275 280 SQATMDERNRQIAEIGASLI AmpR SQATMDERNRQIAEIGASLI g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a

4200 285 KHW* AmpR KHW* t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a

4260 t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a

4320 c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g

4380 ori t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a

4440 ori a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c

4500 ori c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t

4560 ori c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g

4620 ori t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t

4680 ori t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g

4740 ori g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t

4800 ori a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c

4860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c

4920 ori a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c

4980 c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g

5040 t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g

BspQISapI 5100 A G C G A G T C A G T G A G C G A G L4440 t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g

5160 a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c

5220 CAP binding site g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t

5280 -35 -10 CAP binding site lac promoter lac operator A G M13/pUC Reverse t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c

5340 lac operator M13 rev C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c t c t a g c t a g a g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g a g a t c g a t c t

5400 g g t c g a c c a a t t c t c a t g t t t g a c a g c t t a t c a t c g c a g a t c c g g g c a a c g t t g t t g c c a c c a g c t g g t t a a g a g t a c a a a c t g t c g a a t a g t a g c g t c t a g g c c c g t t g c a a c a a c g g t

5460 t t g c t g c a g g c g c a g a a c t g g t a g g t a t g g a a g a t c t a t a c a t t g a a t c a a t a t t g g c a a a a c g a c g t c c g c g t c t t g a c c a t c c a t a c c t t c t a g a t a t g t a a c t t a g t t a t a a c c g t t

5520 t t a g c c a t a t t a g t c a t t g g t t a t a t a g c a t a a a t c a a t a t t g g c t a t t g g c c a t t g c a t a a t c g g t a t a a t c a g t a a c c a a t a t a t c g t a t t t a g t t a t a a c c g a t a a c c g g t a a c g t a

BsrGI 5580 a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t a t t g g c t c a t g t c c a a t a t g a c c g c c a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t a t a a c c g a g t a c a g g t t a t a c t g g c g g t

5640 CMV enhancer t g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a

5700 CMV enhancer a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c

5760 CMV enhancer c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t

5820 CMV enhancer g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t

5880 CMV enhancer c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g

SnaBIBsaAI 5940 CMV enhancer g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a c t t t c c t a c t t g g c a g t a c a t c t a c c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t g a a a g g a t g a a c c g t c a t g t a g a t g

6000 CMV enhancer CMV promoter g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a c c a a t g g g c g t g g a c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t g g t t a c c c g c a c c t

6060 CMV promoter t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c

6120 CMV promoter C G CMV-F t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a t a a c c c c g c c c c g t t g a c g a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t a t t g g g g c g g g g c a a c t g c

6180 CMV promoter C A A A T G G G C G G T A G G C G T G CMV-F A G C T C G T T T A G T G A A C LNCX c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g

6240 C G T C A G A T C LNCX c g t c a g a t c c t c a c t c t c t t c c g c a t c g c t g t c t g c g a g g g c c a g c t g t t g g g c t c g c g g g c a g t c t a g g a g t g a g a g a a g g c g t a g c g a c a g a c g c t c c c g g t c g a c a a c c c g a g c g c c

6300 t t g a g g a c a a a c t c t t c g c g g t c t t t c c a g t a c t c t t g g a t c g g a a a c c c g t c g g c c t c c a a c t c c t g t t t g a g a a g c g c c a g a a a g g t c a t g a g a a c c t a g c c t t t g g g c a g c c g g a g g

6360 g a a c g g t a c t c c g c c a c c g a g g g a c c t g a g c g a g t c c g c a t c g a c c g g a t c g g a a a a c c t c t t g c c a t g a g g c g g t g g c t c c c t g g a c t c g c t c a g g c g t a g c t g g c c t a g c c t t t t g g a

BlpIPaeR7IXhoI 6420 c t c g a g a a a g g c g t c t a a c c a g t c a c a g t c g c a a g g t a g g c t g a g c a c c g t g g c g g g c g g g a g c t c t t t c c g c a g a t t g g t c a g t g t c a g c g t t c c a t c c g a c t c g t g g c a c c g c c c g c c

6480 c a g c g g g t g g c g g t c g g g g t t g t t t c t g g c g g a g g t g c t g c t g a t g a t g t a a t t a a a g t a g t c g c c c a c c g c c a g c c c c a a c a a a g a c c g c c t c c a c g a c g a c t a c t a c a t t a a t t t c a t

BsmBIEsp3I 6540 g g c g g t c t t g a g a c g g c g g a t g g t c g a g g t g a g g t g t g g c a g g c t t g a g a t c c a g c t g t t c c g c c a g a a c t c t g c c g c c t a c c a g c t c c a c t c c a c a c c g t c c g a a c t c t a g g t c g a c a a

6600 g g g g t g a g t a c t c c c t c t c a a a a g c g g g c a t t a c t t c t g c g c t a a g a t t g t c a g t t t c c a c c c c a c t c a t g a g g g a g a g t t t t c g c c c g t a a t g a a g a c g c g a t t c t a a c a g t c a a a g g t

6660 a a a a c g a g g a g g a t t t g a t a t t c a c c t g g c c c g a t c t g g c c a t a c a c t t g a g t g a c a a t g t t t t g c t c c t c c t a a a c t a t a a g t g g a c c g g g c t a g a c c g g t a t g t g a a c t c a c t g t t a c

EagINotIPmeI 6720 T T G C C T T T C T C T C C A C A G G T pMT2-F a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g t c c a a g t t t a a a c t g c g g t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c a g g t t c a a a t t t g a c g c c

6780 Kozak sequence MAAALFVLLGFALLGTH c c g c c a c c a t g g c c g c t g c c c t g t t c g t g c t g c t g g g c t t c g c t c t g c t g g g a a c c c a t g g g c g g t g g t a c c g g c g a c g g g a c a a g c a c g a c g a c c c g a a g c g a g a c g a c c c t t g g g t a c

BmtINheI 6840 GASGAAGFVQAPLSQQRWVG g c g c t a g c g g c g c t g c c g g a t t t g t g c a g g c c c c c c t g a g c c a g c a g a g a t g g g t c g g c g c g c g a t c g c c g c g a c g g c c t a a a c a c g t c c g g g g g g a c t c g g t c g t c t c t a c c c a g c c g c

6900 GSVELHCEAVGSPVPEIQWW g c a g c g t g g a a c t g c a c t g c g a g g c c g t g g g c a g c c c t g t g c c t g a g a t c c a g t g g t g g t c g t c g c a c c t t g a c g t g a c g c t c c g g c a c c c g t c g g g a c a c g g a c t c t a g g t c a c c a c c a

KasINarISfoIPluTI 6960 FEGQGPNDTCSQLWDGARLD t c g a g g g c c a g g g c c c c a a c g a t a c c t g t a g c c a g c t g t g g g a c g g c g c c a g a c t g g a c a a g c t c c c g g t c c c g g g g t t g c t a t g g a c a t c g g t c g a c a c c c t g c c g c g g t c t g a c c t g t

7020 RVHIHATYHQHAASTISIDT g a g t g c a c a t c c a c g c c a c c t a t c a c c a g c a c g c c g c c t c t a c c a t t a g c a t c g a c a c c c c t c a c g t g t a g g t g c g g t g g a t a g t g g t c g t g c g g c g g a g a t g g t a a t c g t a g c t g t g g g

7080 LVEEDTGTYECRASNDPDRN t g g t g g a a g a g g a c a c c g g c a c c t a c g a g t g c a g a g c c a g c a a c g a c c c c g a c c g g a a c c a c c a c c t t c t c c t g t g g c c g t g g a t g c t c a c g t c t c g g t c g t t g c t g g g g c t g g c c t t g g

7140 HLTRAPRVKWVRAQAVVLVL *ATTSTSS a c c t g a c c a g a g c c c c c a g a g t g a a a t g g g t c c g g g c t c a g g c t g t g g t g c t g g t g c t g g t g g a c t g g t c t c g g g g g t c t c a c t t t a c c c a g g c c c g a g t c c g a c a c c a c g a c c a c g a c c

7200 EPGTVFTTVEDLGSKILLTC GPVTNVVTSSRPLLIRSVQL a a c c c g g c a c c g t g t t c a c c a c c g t g g a g g a c c t g g g c a g c a a g a t c c t g c t g a c c t g t a t t g g g c c g t g g c a c a a g t g g t g g c a c c t c c t g g a c c c g t c g t t c t a g g a c g a c t g g a c a t

7260 MAEGRRGA SLNDSATEVTGHRWLKGGVV RFSLAVSTVPWLHSFPPTTS g c c t g a a c g a c a g c g c c a c c g a a g t g a c c g g c c a c a g a t g g c t g a a g g g c g g c g t g g t g c c g g a c t t g c t g t c g c g g t g g c t t c a c t g g c c g g t g t c t a c c g a c t t c c c g c c g c a c c a c g

7320 ERGRPARPENRVQGGQRRPV LKEDALPGQKTEFKVDSDDQ FSSARGPWFVSNLTSLSSWH t g a a a g a g g a c g c c c t g c c c g g c c a g a a a a c c g a g t t c a a g g t g g a c a g c g a c g a c c a g t a c t t t c t c c t g c g g g a c g g g c c g g t c t t t t g g c t c a a g t t c c a c c t g t c g c t g c t g g t c a

7380 GRVQLRVPARAYGHRQYPAA WGEYSCVFLPEPMGTANIQL PSYLQTNRGSGIPVALIWSC g g g g c g a g t a c a g c t g c g t g t t c c t g c c c g a g c c t a t g g g c a c c g c c a a t a t c c a g c t g c c c c c g c t c a t g t c g a c g c a c a a g g a c g g g c t c g g a t a c c c g t g g c g g t t a t a g g t c g a c g

7440 RPSKSEGREVQRAHQRGRDS HGPPRVKAVKSSEHINEGET PGGLTFATFDLSCMLSPSVA a c g g c c c t c c a a g a g t g a a g g c c g t g a a g t c c a g c g a g c a c a t c a a c g a g g g c g a g a c a g t g c c g g g a g g t t c t c a c t t c c g g c a c t t c a g g t c g c t c g t g t a g t t g c t c c c g c t c t g t c

7500 HARVQERERAPCDRLGLVQD AMLVCKSESVPPVTDWAWYK MSTHLLSLTGGTVSQAQYLI c c a t g c t c g t g t g c a a g a g c g a g a g c g t g c c c c c t g t g a c c g a c t g g g c c t g g t a c a a g a g g t a c g a g c a c a c g t t c t c g c t c t c g c a c g g g g g a c a c t g g c t g a c c c g g a c c a t g t t c t

7560 HRQRGQGPDERQREPVLREQ ITDSEDKALMNGSESRFFVS VSLSSLARIFPLSLRNKTLL t c a c c g a c a g c g a g g a c a a g g c c c t g a t g a a c g g c a g c g a g a g c c g g t t c t t c g t g a g c a a g t g g c t g t c g c t c c t g t t c c g g g a c t a c t t g c c g t c g c t c t c g g c c a a g a a g c a c t c g t

7620 QPGQKRAAHREPEHGSRPRP SSQGRSELHIENLNMEADPG LWPLLSSCMSFRFMSASGPW g c a g c c a g g g c a g a a g c g a g c t g c a c a t c g a g a a c c t g a a c a t g g a a g c c g a c c c c g g c c c g t c g g t c c c g t c t t c g c t c g a c g t g t a g c t c t t g g a c t t g t a c c t t c g g c t g g g g c c g g

7680 VQMQRHQQQGLRPGHHHPES QYRCNGTSSKGSDQAIITLR YLHLPVLLLPESWAMMVRLT a g t a c a g a t g c a a c g g c a c c a g c a g c a a g g g c t c c g a c c a g g c c a t c a t c a c c c t g a g a g t c a t g t c t a c g t t g c c g t g g t c g t c g t t c c c g a g g c t g g t c c g g t a g t a g t g g g a c t c t c

BssHIIAscI 7702 AVTPGRA VRSHLGAP RDCRPAG t g c g g t c a c a c c t g g g c g c g c c a c g c c a g t g t g g a c c c g c g c g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.